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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1995-9-19
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pubmed:abstractText |
Genomic DNAs of clinical and environmental isolates of Legionella pneumophila belonging to serogroups 3 and 4 were analyzed by macrorestriction analysis by pulsed-field gel electrophoresis. The restriction enzymes SfiI and NotI allowed easy visual separation of epidemiologically unrelated serogroup 3 strains. Three unrelated serogroup 3 strains that were isolated from different locations were identical by this genome mapping technique. Five unrelated serogroup 4 strains were separable by this technique. The electrophoretic patterns obtained after SfiI or NotI cleavage of the DNA of strains isolated from four patients with hospital-acquired legionellosis were identical to the patterns of strains isolated from the hot water supply systems of the buildings in which the patients were hospitalized. In conclusion, macrorestriction analysis is a valuable tool for epidemiological studies of infections caused by L. pneumophila serogroups 3 and 4.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7646036-1320629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7646036-1369446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7646036-1890182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7646036-2179256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7646036-3053773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7646036-3321265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7646036-3517064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7646036-4030689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7646036-6761358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7646036-7852558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7646036-8237978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7646036-8370750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7646036-8394380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7646036-8476497
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0099-2240
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
61
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2000-3
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7646036-Adult,
pubmed-meshheading:7646036-Aged,
pubmed-meshheading:7646036-Cross Infection,
pubmed-meshheading:7646036-DNA, Bacterial,
pubmed-meshheading:7646036-Deoxyribonucleases, Type II Site-Specific,
pubmed-meshheading:7646036-Electrophoresis, Gel, Pulsed-Field,
pubmed-meshheading:7646036-Equipment Contamination,
pubmed-meshheading:7646036-Female,
pubmed-meshheading:7646036-Germany, West,
pubmed-meshheading:7646036-Humans,
pubmed-meshheading:7646036-Legionella pneumophila,
pubmed-meshheading:7646036-Legionnaires' Disease,
pubmed-meshheading:7646036-Male,
pubmed-meshheading:7646036-Middle Aged,
pubmed-meshheading:7646036-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:7646036-Serotyping,
pubmed-meshheading:7646036-Water Microbiology,
pubmed-meshheading:7646036-Water Supply
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pubmed:year |
1995
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pubmed:articleTitle |
DNA polymorphisms in strains of Legionella pneumophila serogroups 3 and 4 detected by macrorestriction analysis and their use for epidemiological investigation of nosocomial legionellosis.
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pubmed:affiliation |
Institut für Medizinische Mikrobiologie und Hygiene, Technische Universität Dresden, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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