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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6540
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pubmed:dateCreated |
1995-9-14
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pubmed:abstractText |
Tyrosine kinase receptors stimulate the Ras signalling pathway by enhancing the activity of the SOS nucleotide-exchange factor. This occurs, at least in part, by the recruitment of an SOS-GRB2 complex to Ras in the plasma membrane. Here we describe a different signalling pathway to Ras that involves activation of the Ras-GRF exchange factor in response to Ca2+ influx. In particular, we show that the ability of Ras-GRF to activate Ras in vivo is markedly enhanced by raised Ca2+ concentrations. Activation is mediated by calmodulin binding to an IQ motif in Ras-GRF, because substitutions in conserved amino acids in this motif prevent both calmodulin binding to Ras-GRF and Ras-GRF activation in vivo. So far, full-length Ras-GRF has been detected only in brain neurons. Our findings implicate Ras-GRF in the regulation of neuronal functions that are influenced by Ca2+ signals.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Brain-Derived Neurotrophic Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/Calmodulin,
http://linkedlifedata.com/resource/pubmed/chemical/Guanine Nucleotide Exchange Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Ionomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/ras Guanine Nucleotide Exchange...,
http://linkedlifedata.com/resource/pubmed/chemical/ras Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/ras-GRF1
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0028-0836
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
376
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
524-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7637786-Amino Acid Sequence,
pubmed-meshheading:7637786-Animals,
pubmed-meshheading:7637786-Brain-Derived Neurotrophic Factor,
pubmed-meshheading:7637786-Calcium,
pubmed-meshheading:7637786-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:7637786-Calmodulin,
pubmed-meshheading:7637786-Cell Line,
pubmed-meshheading:7637786-Cells, Cultured,
pubmed-meshheading:7637786-Cerebral Cortex,
pubmed-meshheading:7637786-Enzyme Activation,
pubmed-meshheading:7637786-Guanine Nucleotide Exchange Factors,
pubmed-meshheading:7637786-Ionomycin,
pubmed-meshheading:7637786-Membrane Potentials,
pubmed-meshheading:7637786-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:7637786-Mitogen-Activated Protein Kinases,
pubmed-meshheading:7637786-Molecular Sequence Data,
pubmed-meshheading:7637786-Nerve Tissue Proteins,
pubmed-meshheading:7637786-Neurons,
pubmed-meshheading:7637786-Protein Binding,
pubmed-meshheading:7637786-Proteins,
pubmed-meshheading:7637786-Rats,
pubmed-meshheading:7637786-Signal Transduction,
pubmed-meshheading:7637786-Transfection,
pubmed-meshheading:7637786-ras Guanine Nucleotide Exchange Factors,
pubmed-meshheading:7637786-ras Proteins,
pubmed-meshheading:7637786-ras-GRF1
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pubmed:year |
1995
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pubmed:articleTitle |
Calcium activation of Ras mediated by neuronal exchange factor Ras-GRF.
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pubmed:affiliation |
Department of Biochemistry, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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