Linked Life Data

7629107

Source:http://linkedlifedata.com/resource/pubmed/id/7629107

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
30
pubmed:dateCreated
1995-9-5
pubmed:databankReference
pubmed:abstractText
5-Lipoxygenase of mouse macrophages and bone marrow-derived mast cells (BMMC) was investigated. Indirect immunocytofluorescence combined with confocal microscopy provided evidence for distinct intracellular expression patterns and trafficking of 5-lipoxygenase upon cellular activation. In resting BMMC, 5-lipoxygenase was found within the nucleus co-localizing with the nuclear stain Yo-Pro-1. When BMMC were IgE/antigen-activated the 5-lipoxygenase immunofluorescence pattern was changed from nuclear to perinuclear. The absence of divalent cations in the incubation medium, or calcium ionophore A23187 challenge, altered the predominantly nuclear expression pattern to new sites both cytosolic and intranuclear. The cDNA for murine macrophage 5-lipoxygenase was cloned by the polymerase chain reaction and would predict a 674 amino acid protein. Using control cells obtained from 5-lipoxygenase-deficient mice it was determined that a single isoform accounts for both soluble and membrane-bound and nuclear and cytosolic-localized enzyme in macrophages and BMMC. A mutation at amino acid 672 (Val-->Met) introduced serendipitously during the cloning process was found to completely abolish 5-lipoxygenase enzyme activity when the enzyme was expressed in human embryonic kidney 293 cells. This subtle change is proposed to affect the ability of the COOH-terminal isoleucine to coordinate the essential non-heme iron atom. In macrophages and BMMC obtained from 5-lipoxygenase-deficient mice, compensatory changes in expression of genes involved in the biosynthesis of leukotriene B4 were investigated. 5-Lipoxygenase-activating protein expression was reduced by 50%, while leukotriene A4 hydrolase expression was unaltered. The 5-lipoxygenase gene was mapped to the central region of mouse chromosome 6 in a region that shares homology with human chromosome 10 by interspecific backcross analysis. These studies provide a global picture of the murine 5-lipoxygenase system and raise questions about the role of 5-lipoxygenase and leukotrienes within the nucleus.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
270
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
17993-9
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:7629107-5-Lipoxygenase-Activating Proteins, pubmed-meshheading:7629107-Animals, pubmed-meshheading:7629107-Arachidonate 5-Lipoxygenase, pubmed-meshheading:7629107-Base Sequence, pubmed-meshheading:7629107-Bone Marrow, pubmed-meshheading:7629107-Bone Marrow Cells, pubmed-meshheading:7629107-Carrier Proteins, pubmed-meshheading:7629107-Cell Line, pubmed-meshheading:7629107-Chromosome Mapping, pubmed-meshheading:7629107-Cloning, Molecular, pubmed-meshheading:7629107-DNA, Complementary, pubmed-meshheading:7629107-DNA Primers, pubmed-meshheading:7629107-Epoxide Hydrolases, pubmed-meshheading:7629107-Humans, pubmed-meshheading:7629107-Macrophages, Alveolar, pubmed-meshheading:7629107-Membrane Proteins, pubmed-meshheading:7629107-Mice, pubmed-meshheading:7629107-Mice, Inbred C57BL, pubmed-meshheading:7629107-Molecular Sequence Data, pubmed-meshheading:7629107-Mutagenesis
pubmed:year
1995
pubmed:articleTitle
cDNA cloning, expression, mutagenesis, intracellular localization, and gene chromosomal assignment of mouse 5-lipoxygenase.
pubmed:affiliation
Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.