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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1995-8-30
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pubmed:abstractText |
C-ANCA, which are directed against neutrophil proteinase 3 (PR3), are specific markers for the diagnosis of active Wegener's granulomatosis (WG). The correlation between C-ANCA titre and WG disease activity suggests that these autoantibodies are involved in the development of WG. We have previously observed that C-ANCA interfere with PR3 proteolytic activity and with complexation of PR3 with its major physiologic inhibitor alpha 1-antitrypsin (alpha 1-AT). The possible pathogenic importance of C-ANCA may be related to the stability of C-ANCA IgG-PR3 complexes. In the present study we tested proteolysis by PR3 of human IgG and proteolysis of C-ANCA IgG complexed to the enzyme. All human IgG subclass proteins were cleaved by PR3. Digestion products were compared with those obtained by human neutrophil elastase (HNE)-mediated proteolysis of human IgG subclass proteins. Although cleavage products of similar size could be identified, the proteolytic activity of both enzymes towards human IgG differed. Furthermore, inhibiting C-ANCA IgG were cleaved into small peptides when complexed to PR3. The possible pathogenic consequences of these findings will be discussed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-12412739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-1458677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-1551417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-1647776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-1677531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-1688612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-1975893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-2033050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-2161532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-2189509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-2202308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-2258701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-2312733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-2501794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-2660645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-2679910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-2681270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-2684074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-2857806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-3049904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-3198760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-637870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-8370167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7621597-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
101
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8-12
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7621597-Antibodies, Antineutrophil Cytoplasmic,
pubmed-meshheading:7621597-Antigen-Antibody Complex,
pubmed-meshheading:7621597-Autoantibodies,
pubmed-meshheading:7621597-Humans,
pubmed-meshheading:7621597-Hydrolysis,
pubmed-meshheading:7621597-Immunoglobulin G,
pubmed-meshheading:7621597-Myeloblastin,
pubmed-meshheading:7621597-Peptide Fragments,
pubmed-meshheading:7621597-Proteins,
pubmed-meshheading:7621597-Serine Endopeptidases
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pubmed:year |
1995
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pubmed:articleTitle |
Proteolysis of classic anti-neutrophil cytoplasmic autoantibodies (C-ANCA) by neutrophil proteinase 3.
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pubmed:affiliation |
Central Laboratory, Netherlands Red Cross Blood Transfusion Service, Amsterdam.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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