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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1995-8-18
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pubmed:abstractText |
The proteins encoded by the human papillomavirus type 16 E6-E7 open reading frame are essential for transformation of the host cell. Two mRNA species, E6*I and E6*II, generated by alternative splicing of a polycistronic pre-mRNA, encode truncated E6 proteins and the E7 protein. Our investigation assessed whether or not the level of expression of E6*I and E6*II varies quantitatively in relation to the grade of cervical intraepithelial neoplasia (CIN). We used a quantitative reverse transcription PCR assay to quantify these transcripts in concurrently collected biopsy tissue and exfoliated cervical cells from 22 women with a normal cervix or various grades of CIN. We evaluated transcription profiles in relation to CIN grade and specimen type. The expression levels of E6*I and E6*II in exfoliated cervical cells did not vary significantly in relation to the grade of CIN. However, expression of E6*II was significantly diminished or absent in biopsy tissue obtained from CIN grade II and III lesions (P = 0.014). Our findings suggest that quantification of E6*I and E6*II expression in biopsy tissue may be more clinically relevant than analysis of exfoliated cells. The identification of distinct patterns of expression in association with low- and high-grade CIN suggests that quantification of E6*I and E6*II expression in biopsy tissue may have prognostic value as an indicator of CIN progression.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-1310488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-1314365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-1321226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-1653501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-1667790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-1691205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-1695672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-1727369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-2106330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-2161056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-2167553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-2536845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-2552162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-2983228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-2999980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-3014503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-3029430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-3036495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-3683388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-3748500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-6096821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-7690739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-8228915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615724-8244576
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/E6 protein, Human papillomavirus...,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Papillomavirus E7 Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/oncogene protein E7, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
33
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1169-73
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:7615724-Base Sequence,
pubmed-meshheading:7615724-Carcinoma in Situ,
pubmed-meshheading:7615724-DNA Primers,
pubmed-meshheading:7615724-Female,
pubmed-meshheading:7615724-Gene Expression,
pubmed-meshheading:7615724-Humans,
pubmed-meshheading:7615724-Molecular Sequence Data,
pubmed-meshheading:7615724-Oncogene Proteins, Viral,
pubmed-meshheading:7615724-Open Reading Frames,
pubmed-meshheading:7615724-Papillomaviridae,
pubmed-meshheading:7615724-Papillomavirus E7 Proteins,
pubmed-meshheading:7615724-Prognosis,
pubmed-meshheading:7615724-RNA, Messenger,
pubmed-meshheading:7615724-RNA, Viral,
pubmed-meshheading:7615724-Repressor Proteins,
pubmed-meshheading:7615724-Uterine Cervical Neoplasms
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pubmed:year |
1995
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pubmed:articleTitle |
Expression of human papillomavirus type 16 E6-E7 open reading frame varies quantitatively in biopsy tissue from different grades of cervical intraepithelial neoplasia.
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pubmed:affiliation |
Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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