Linked Life Data

7605830

Source:http://linkedlifedata.com/resource/pubmed/id/7605830

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-8-17
pubmed:abstractText
We have evaluated a new turbidimetric immunoassay test (On Line, Roche) for the quantification of digoxin in human serum without sample pretreatment. The assay, automated on a Cobas-Mira clinical analyser, is based on the measurement of the changes in absorbance that occur when digoxin-coated microparticles aggregate with digoxin antibody; the aggregation reaction is partially inhibited by digoxin in the sample. The calibration curve extends up to 6.4 nmol/l, with a wide measuring range, and was stable at least for 10 days during the studied period. The intra- and inter-assay coefficients of variation were in all cases lower than 7%. The detection limit of the assay was 0.256 nmol/l and the linearity, assessed by measuring serial dilutions of poisoned patient sera, showed correlation coefficients higher than 0.998. There is no interference from bilirubin (up to 340 mumol/l) or haemoglobin (up to 7900 mg/l), although a positive interference by lipids was found, which can be avoided by previous delipidation of these sera. No digoxin-like factors were identified affecting digoxin values in a concentration higher than that of the sensitivity of the assay in samples of premature infants, pregnant women, or patients with renal or hepatic failure. Correlation coefficients between the turbidimetric assay and two immunoassays (fluorescence polarization and RIA) were 0.983 and 0.955 respectively, calculated from the assay of 102 and 98 samples. Nevertheless the values obtained by the evaluated assay were proportionally 20% higher, a fact probably related to differences in calibrators.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0939-4974
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
171-5
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Evaluation of an immunoturbidimetric assay of serum digoxin without sample pretreatment.
pubmed:affiliation
Laboratorio de Bioquímica, Hospital San Millán, Logroño, La Rioja, Spain.
pubmed:publicationType
Journal Article