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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
1995-8-3
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pubmed:abstractText |
Retrovirus-mediated gene transfer into hepatocytes in vivo results in long-term gene expression. Limitations include the need to remove two-thirds of the liver and the relatively low frequency of gene transfer. To increase gene transfer without surgical hepatectomy, mouse hepatocytes were transduced in vivo with a recombinant adenovirus that transiently expressed urokinase, resulting in high rates of asynchronous liver regeneration. During the regenerative phase, in vivo retroviral-mediated gene transfer in hepatocytes resulted in 5- to 10-fold greater transduction efficiencies than that obtained by conventional partial hepatectomy. In 3-4 weeks, the architecture and microscopic structure of the recipient livers were normal. The two-viral system of achieving permanent transgene expression from hepatocytes in vivo offers an alternative approach to current ex vivo and in vivo gene-transfer models.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-1334458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-1482704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-1644927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-1650585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-1656443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-1696178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-1713128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-1722351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-1729724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-2170370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-2536987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-2965450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-3139404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-3143380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-6360818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-6682680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-7703287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-7719932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-7875680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-7980740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-7981306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-8015304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-8054972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-8108431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-8134398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-8183921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-8211118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-8362987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-8382843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-8396259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-8399487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7597103-8399488
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
92
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6210-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7597103-Adenoviridae,
pubmed-meshheading:7597103-Animals,
pubmed-meshheading:7597103-Cells, Cultured,
pubmed-meshheading:7597103-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:7597103-Female,
pubmed-meshheading:7597103-Gene Transfer Techniques,
pubmed-meshheading:7597103-Genetic Vectors,
pubmed-meshheading:7597103-Humans,
pubmed-meshheading:7597103-Liver,
pubmed-meshheading:7597103-Liver Regeneration,
pubmed-meshheading:7597103-Mice,
pubmed-meshheading:7597103-Mice, Inbred C57BL,
pubmed-meshheading:7597103-Time Factors,
pubmed-meshheading:7597103-Urokinase-Type Plasminogen Activator,
pubmed-meshheading:7597103-alpha 1-Antitrypsin,
pubmed-meshheading:7597103-beta-Galactosidase
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pubmed:year |
1995
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pubmed:articleTitle |
Adenovirus-mediated urokinase gene transfer induces liver regeneration and allows for efficient retrovirus transduction of hepatocytes in vivo.
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pubmed:affiliation |
Markey Molecular Medicine Center, Department of Medicine, University of Washington, Seattle 98195, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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