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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1995-12-7
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pubmed:abstractText |
Involvement of tyrosine phosphorylation in osteoclastic bone resorption was examined using osteoclast-like multinucleated cells prepared from co-cultures of mouse osteoblastic cells and bone marrow cells in the presence of 1 alpha,25-dihydroxyvitamin D3. When osteoclast-like cells were plated on culture dishes in the presence of 10% fetal bovine serum, they were sharply stained in their peripheral region by anti-phosphotyrosine antibody. Western blot analysis revealed that 115- to 130-kD proteins were tyrosine-phosphorylated in osteoclast-like cells. Using immunoprecipitation and immunoblotting, one of the proteins with 115-130 kD was identified as focal adhesion kinase (p125FAK), a tyrosine kinase, which is localized in focal adhesions. Immunostaining with anti-p125FAK antibody revealed that p125FAK was mainly localized at the periphery of osteoclast-like cells. Herbimycin A, a tyrosine kinase inhibitor, not only suppressed tyrosine phosphorylation of p125FAK but also changed the intracellular localization of p125FAK and disrupted a ringed structure of F-actin-containing podosomes in osteoclast-like cells. Antisense oligodeoxynucleotides to p125FAK inhibited dentine resorption by osteoclast-like cells, whereas sense oligodeoxynucleotides did not. These results suggest that p125FAK is involved in osteoclastic bone resorption and that tyrosine phosphorylation of p125FAK is critical for regulating osteoclast function.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Benzoquinones,
http://linkedlifedata.com/resource/pubmed/chemical/Calcitriol,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Focal Adhesion Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Focal Adhesion Protein-Tyrosine...,
http://linkedlifedata.com/resource/pubmed/chemical/Lactams, Macrocyclic,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Ptk2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Quinones,
http://linkedlifedata.com/resource/pubmed/chemical/Thionucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/herbimycin
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0730-2312
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
58
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
424-35
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7593264-Animals,
pubmed-meshheading:7593264-Animals, Newborn,
pubmed-meshheading:7593264-Base Sequence,
pubmed-meshheading:7593264-Benzoquinones,
pubmed-meshheading:7593264-Bone Marrow Cells,
pubmed-meshheading:7593264-Bone Resorption,
pubmed-meshheading:7593264-Calcitriol,
pubmed-meshheading:7593264-Cattle,
pubmed-meshheading:7593264-Cell Adhesion Molecules,
pubmed-meshheading:7593264-Cells, Cultured,
pubmed-meshheading:7593264-Coculture Techniques,
pubmed-meshheading:7593264-Dentin,
pubmed-meshheading:7593264-Enzyme Inhibitors,
pubmed-meshheading:7593264-Focal Adhesion Kinase 1,
pubmed-meshheading:7593264-Focal Adhesion Protein-Tyrosine Kinases,
pubmed-meshheading:7593264-Hematopoietic Stem Cells,
pubmed-meshheading:7593264-Immunohistochemistry,
pubmed-meshheading:7593264-Lactams, Macrocyclic,
pubmed-meshheading:7593264-Male,
pubmed-meshheading:7593264-Mice,
pubmed-meshheading:7593264-Mice, Inbred Strains,
pubmed-meshheading:7593264-Molecular Sequence Data,
pubmed-meshheading:7593264-Oligodeoxyribonucleotides,
pubmed-meshheading:7593264-Oligonucleotides, Antisense,
pubmed-meshheading:7593264-Osteoclasts,
pubmed-meshheading:7593264-Phosphoproteins,
pubmed-meshheading:7593264-Phosphotyrosine,
pubmed-meshheading:7593264-Protein-Tyrosine Kinases,
pubmed-meshheading:7593264-Quinones,
pubmed-meshheading:7593264-Thionucleotides
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pubmed:year |
1995
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pubmed:articleTitle |
Possible involvement of focal adhesion kinase, p125FAK, in osteoclastic bone resorption.
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pubmed:affiliation |
Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan.
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pubmed:publicationType |
Journal Article
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