Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
44
|
| pubmed:dateCreated |
1995-12-21
|
| pubmed:abstractText |
Glucocorticoids regulate both bone formation and bone resorption. In osteoblasts, they inhibit type I collagen synthesis; however, there is limited information about their effects on interstitial collagenase, the enzyme that degrades type I collagen. We used primary cultures of osteoblast-enriched cells from fetal rat calvariae (Ob cells) to study the effects of cortisol on collagenase expression. Northern blot analysis showed that cortisol increased collagenase transcript levels in a dose- and time-dependent manner, which was paralleled by an increase in immunoreactive metalloproteinase in the culture medium. Cortisol increased the half-life of collagenase mRNA from 6 to 12 h in transcription-arrested Ob cells. In contrast, cortisol modestly decreased collagenase gene transcription after 24 h of treatment. The up-regulation of collagenase by cortisol is osteoblast-specific, since the glucocorticoid decreased phorbol 12-myristate 13-acetate-induced collagenase mRNA expression in rat fibroblasts, a result that agrees with other studies of collagenase gene regulation in fibroblastic cells. In conclusion, cortisol increases interstitial collagenase transcript levels by post-transcriptional mechanisms in osteoblastic cells. Our data demonstrate that glucocorticoids regulate collagenase gene expression in a novel tissue-specific manner, further highlighting the differences in gene regulation between osteoblastic and fibroblastic cells.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Collagenases,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrocortisone,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
3
|
| pubmed:volume |
270
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
26607-12
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7592884-Animals,
pubmed-meshheading:7592884-Blotting, Northern,
pubmed-meshheading:7592884-Cells, Cultured,
pubmed-meshheading:7592884-Collagenases,
pubmed-meshheading:7592884-Fetus,
pubmed-meshheading:7592884-Fibroblasts,
pubmed-meshheading:7592884-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:7592884-Hydrocortisone,
pubmed-meshheading:7592884-Kinetics,
pubmed-meshheading:7592884-Luciferases,
pubmed-meshheading:7592884-Matrix Metalloproteinase 1,
pubmed-meshheading:7592884-Organ Specificity,
pubmed-meshheading:7592884-Osteoblasts,
pubmed-meshheading:7592884-RNA, Messenger,
pubmed-meshheading:7592884-RNA Processing, Post-Transcriptional,
pubmed-meshheading:7592884-Rats,
pubmed-meshheading:7592884-Recombinant Proteins,
pubmed-meshheading:7592884-Skull,
pubmed-meshheading:7592884-Tetradecanoylphorbol Acetate,
pubmed-meshheading:7592884-Time Factors,
pubmed-meshheading:7592884-Transcription, Genetic,
pubmed-meshheading:7592884-Transfection
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Cortisol increases interstitial collagenase expression in osteoblasts by post-transcriptional mechanisms.
|
| pubmed:affiliation |
Department of Research, Saint Francis Hospital and Medical Center, Hartford, Connecticut 06105, USA.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
|