Linked Life Data

7589890

Source:http://linkedlifedata.com/resource/pubmed/id/7589890

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-12-7
pubmed:abstractText
To begin to determine the role of the alpha 2 beta 1 integrin and its ligands, collagen I, collagen IV, and laminin, in mammary epithelial differentiation in vivo, we determined the expression of these molecules by in situ hybridization and immunofluorescence in the developing mouse mammary gland. Expression of collagen I, collagen IV, and laminin mRNAs in the mammary gland during puberty corresponded to the period of greatest growth of the gland, 4-7 weeks postnatally. Collagen I expression preceded collagen IV expression, both of which preceded laminin expression, suggesting an important temporal sequence of extracellular matrix (ECM) production. When growth of the epithelium ceased in the adult virgin gland, expression of all three mRNAs became undetectable. Following the onset of pregnancy these molecules were re-expressed with the same chronology observed during puberty. Collagen I, collagen IV, and laminin were expressed by stromal cells immediately surrounding the developing ductal epithelium. Surprisingly, we found no expression of ECM components in the epithelial cells, suggesting the mammary epithelium does not synthesize its own basement membrane. The distribution of collagen I was consistent with a role in duct formation, since collagen I was strikingly abundant around larger mammary ducts, but was sparse around growing endbuds or alveoli. Conversely, there was abundant laminin near growing endbuds and around alveoli, and less around large ducts, suggesting its role is different than collagen I. The alpha 2 beta 1 integrin was present on the basal, lateral, and apical surfaces of the mammary epithelium throughout postnatal development and pregnancy. The alpha 2 beta 1 integrin expression was strongest at midpregnancy, suggesting a role for alpha 2 beta 1 integrin in the alveolar formation that occurs at this time. The alpha 2 beta 1 integrin expression decreased dramatically in the lactating gland. Our results suggest that alpha 2 beta 1 integrin interactions with its temporally and spatially regulated ligands, collagen I, collagen IV, and laminin, could play an important role in mammary morphogenesis in vivo.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0301-4681
pubmed:author
pubmed:issnType
Print
pubmed:volume
59
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-13
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:7589890-Animals, pubmed-meshheading:7589890-Basement Membrane, pubmed-meshheading:7589890-Collagen, pubmed-meshheading:7589890-Connective Tissue, pubmed-meshheading:7589890-Epithelial Cells, pubmed-meshheading:7589890-Epithelium, pubmed-meshheading:7589890-Extracellular Matrix, pubmed-meshheading:7589890-Female, pubmed-meshheading:7589890-Gene Expression, pubmed-meshheading:7589890-In Situ Hybridization, pubmed-meshheading:7589890-Integrins, pubmed-meshheading:7589890-Laminin, pubmed-meshheading:7589890-Mammary Glands, Animal, pubmed-meshheading:7589890-Mice, pubmed-meshheading:7589890-Mice, Inbred BALB C, pubmed-meshheading:7589890-Morphogenesis, pubmed-meshheading:7589890-Pregnancy, pubmed-meshheading:7589890-RNA, Messenger, pubmed-meshheading:7589890-Rabbits, pubmed-meshheading:7589890-Receptors, Collagen, pubmed-meshheading:7589890-Sexual Maturation, pubmed-meshheading:7589890-Stromal Cells
pubmed:year
1995
pubmed:articleTitle
The spatial and temporal expression of the alpha 2 beta 1 integrin and its ligands, collagen I, collagen IV, and laminin, suggest important roles in mouse mammary morphogenesis.
pubmed:affiliation
Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110-1093, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't