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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1995-12-8
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pubmed:abstractText |
We studied the hematopoietic activity of peripheral blood stem cells (PBSC) mobilized by recombinant human granulocyte colony-stimulating factor (G-CSF) using semisolid and long-term culture systems (LTC) in 5 normal individuals. Following 2 or 3 daily subcutaneous injections of G-CSF (filgrastim; 1.5 micrograms/kg), not only committed progenitors including CFU-GM, BFU-E, and CFU-Mix, but also long-term culture initiating cells (LTC-IC) were increased in the peripheral blood. When the cells derived from CFU-GM, BFU-E and CFU-Mix colonies were replated for secondary colony formation, a minor fraction of CFU-GM and CFU-Mix colonies formed after 3 days of G-CSF stimulation in vivo could produce secondary colonies. Moreover, the replating capacity of primary colonies from 5-week-old LTC initiated after 3 days of G-CSF stimulation was increased compared to that from 5-week-old LTC initiated in a 'steady-state'. These observations indicate that G-CSF can mobilize very primitive progenitors (LTC-IC) along with committed progenitors into the circulation and suggest that some of these G-CSF-mobilized progenitors may retain self-renewal capacity.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0268-3369
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
57-62
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7581129-Adult,
pubmed-meshheading:7581129-Cell Differentiation,
pubmed-meshheading:7581129-Cells, Cultured,
pubmed-meshheading:7581129-Colony-Forming Units Assay,
pubmed-meshheading:7581129-Female,
pubmed-meshheading:7581129-Granulocyte Colony-Stimulating Factor,
pubmed-meshheading:7581129-Hematopoietic Stem Cells,
pubmed-meshheading:7581129-Humans,
pubmed-meshheading:7581129-Injections, Subcutaneous,
pubmed-meshheading:7581129-Male,
pubmed-meshheading:7581129-Middle Aged,
pubmed-meshheading:7581129-Recombinant Proteins
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pubmed:year |
1995
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pubmed:articleTitle |
Granulocyte colony-stimulating factor mobilizes primitive hematopoietic stem cells in normal individuals.
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pubmed:affiliation |
First Department of Internal Medicine, Faculty of Medicine, Fukuoka, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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