Linked Life Data

7576546

Source:http://linkedlifedata.com/resource/pubmed/id/7576546

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1995-12-4
pubmed:abstractText
Strain 2-79 is a biocontrol agent against take-all, an important disease of wheat caused by Gaeumannomyces graminis var. tritici. In the rhizosphere, it produces the antibiotic phenazine 1-carboxylic acid (PCA) as the primary means of disease suppression. One barrier to commercial use of phenazine-producing pseudomonads, like strain 2-79, is the lack of liquid-culture technology for mass production. For instance, there is little published research concerning the impact of liquid-culture secondary metabolism on the biocontrol qualities of the cell harvest, i.e., efficacy, phytotoxicity, and storage survival. Yet it is important to know whether the fermentation process should be designed to enhance or eliminate secondary metabolite accumulation. To enable future exploration of this issue, we identified liquid-culture parameters that could be manipulated to control the phenazine productivity of strain 2-79. Our results indicated that PCA accumulation was very sensitive to the culture pH and temperature. It was possible to produce large cell populations with either high or low phenazine productivity by choosing to control culture pH at 7 and 8 respectively. Although high cell accumulations were achieved over the broad 25-34 degrees C range studied, high, moderate, or low PCA productivities were observed at 25-27 degrees C, 29-32.5 degrees C, or 34 degrees C respectively. When pH was controlled at 7, specific PCA productions at 25 degrees C could be modulated by the choice of carbon source supplied. PCA accumulation per unit biomass reached 0.31 g/g on glucose, 0.16 g/g on glycerol and xylose, and only 0.09 g/g on fructose.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
B
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0175-7598
pubmed:author
pubmed:issnType
Print
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
794-800
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Liquid-culture pH, temperature, and carbon (not nitrogen) source regulate phenazine productivity of the take-all biocontrol agent Pseudomonas fluorescens 2-79.
pubmed:affiliation
Fermentation Biochemistry Research Unit, USDA, Peoria, IL 61604, USA.
pubmed:publicationType
Journal Article