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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1995-5-11
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pubmed:abstractText |
This study was undertaken to determine whether transforming growth factor alpha (TGF-alpha) regulates the production of mouse placental lactogen I (mPL-I) and mPL-II in a manner that is similar to that of epidermal growth factor (EGF), which was previously shown to stimulate mPL-I secretion and inhibit mPL-II secretion. In contrast to the activity of EGF, human (h) and rat (r) TGF-alpha (each at 100 ng/ml) inhibited secretion of mPL-I by placental cells isolated from mice on day 7 of pregnancy. Maximum inhibition of mPL-I secretion occurred on the third day of a 5-day culture period and ranged between 37% and 56% in multiple trials. Incubation of cells with hTGF-alpha and EGF was not followed by a change in the mPL-I concentration of the medium, suggesting the peptides antagonized each other's effects. hTGF-alpha and rTGF-alpha inhibited secretion of mPL-II; maximum inhibition ranged between 62% and 84% in multiple trials. The lowest concentrations of hTGF-alpha that affected mPL-I and mPL-II secretion were 10 ng/ml and 1 ng/ml, respectively. EGF and hTGF-alpha bound to the same receptors on placental cells, as assessed by cross-linking, and both peptides stimulated receptor phosphorylation, as assessed by Western blot analysis. There are three types of mPL-containing cells in placental cultures: cells that contain only mPL-I, cells that contain only mPL-II, and cells that contain both mPLs. The percentage of each type of mPL-containing cell in the culture was determined by immunostaining. hTGF-alpha affected the differentiation of the subpopulations of PL-containing cells in a manner that differed from that of EGF. The data suggest that TGF-alpha and EGF do not regulate the production of mPL-I and mPL-II in a similar manner.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-1396305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-1420315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-1454826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-1546559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-1576257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-1686188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-1733194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-1740693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-1760995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-1777504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-1933884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-2284889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-2302227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-2422759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-2658990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-2788651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-2790004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-2791990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-3032416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-3032595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-3197641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-3211146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-3485799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-3497258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-3497713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-3874924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-3877079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-5544593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-6950428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-7056217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-7316186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-8167219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7535931-8265646
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Placental Lactogen,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/placental lactogen I, rat,
http://linkedlifedata.com/resource/pubmed/chemical/placental lactogen II
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
92
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2830-4
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7535931-Animals,
pubmed-meshheading:7535931-Blotting, Western,
pubmed-meshheading:7535931-Cells, Cultured,
pubmed-meshheading:7535931-Epidermal Growth Factor,
pubmed-meshheading:7535931-Female,
pubmed-meshheading:7535931-Humans,
pubmed-meshheading:7535931-Immunohistochemistry,
pubmed-meshheading:7535931-Kinetics,
pubmed-meshheading:7535931-Mice,
pubmed-meshheading:7535931-Phosphotyrosine,
pubmed-meshheading:7535931-Placenta,
pubmed-meshheading:7535931-Placental Lactogen,
pubmed-meshheading:7535931-Pregnancy,
pubmed-meshheading:7535931-Rats,
pubmed-meshheading:7535931-Receptor, Epidermal Growth Factor,
pubmed-meshheading:7535931-Recombinant Proteins,
pubmed-meshheading:7535931-Transforming Growth Factor alpha,
pubmed-meshheading:7535931-Tyrosine
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pubmed:year |
1995
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pubmed:articleTitle |
Opposite effects of transforming growth factor alpha and epidermal growth factor on mouse placental lactogen I secretion.
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pubmed:affiliation |
Department of Biology, University of California, Santa Cruz 95064, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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