Linked Life Data

7533195

Source:http://linkedlifedata.com/resource/pubmed/id/7533195

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-4-4
pubmed:abstractText
A method for the rapid isolation of functionally active analogues of human CD59 from erythrocytes (E) is described. The method, here applied to pig E, is based on the fractionation of a butanol extract of E ghosts by preparative SDS-PAGE followed by gel filtration on Superose 12. Purification was monitored using a functional complement inhibition assay. SDS-PAGE analysis of the product of this procedure indicated a single protein band with apparent M(r) of 20 kDa under reducing and non-reducing conditions. The preparation could be incorporated into guinea pig E to inhibit both CVF-reactive lysis and lysis through C8 and C9 using preformed C5b-7 sites, demonstrating that it contained a CD59-like activity. PIPLC treatment of the isolated protein abolished the inhibition. In contrast to SDS-PAGE analysis, amino-terminal sequence analysis of the preparation showed that it comprised two components. One was identified from databank searches as a fragment of pig glycophorin. These two components could not be separated by either standard or affinity chromatographic techniques. The second component was novel and had high sequence homology with human CD59, identifying it as the pig analogue. Further functional studies showed that the pig analogue of human CD59 was effective in the protection of guinea pig E against lysis by serum from a variety of species, including human.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
179
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
223-31
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7533195-Amino Acid Sequence, pubmed-meshheading:7533195-Animals, pubmed-meshheading:7533195-Antigens, CD, pubmed-meshheading:7533195-Antigens, CD59, pubmed-meshheading:7533195-Cobra Venoms, pubmed-meshheading:7533195-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:7533195-Erythrocyte Membrane, pubmed-meshheading:7533195-Glycosylphosphatidylinositols, pubmed-meshheading:7533195-Humans, pubmed-meshheading:7533195-Membrane Glycoproteins, pubmed-meshheading:7533195-Molecular Sequence Data, pubmed-meshheading:7533195-Phosphatidylinositol Diacylglycerol-Lyase, pubmed-meshheading:7533195-Phosphoric Diester Hydrolases, pubmed-meshheading:7533195-Rats, pubmed-meshheading:7533195-Sequence Alignment, pubmed-meshheading:7533195-Sequence Homology, Amino Acid, pubmed-meshheading:7533195-Swine
pubmed:year
1995
pubmed:articleTitle
A rapid method for the isolation of analogues of human CD59 by preparative SDS-PAGE: application to pig CD59.
pubmed:affiliation
Department of Medical Biochemistry, University of Wales College of Medicine, Heath Park, Cardiff, UK.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't