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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1995-3-16
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pubmed:abstractText |
A new human carcinoma cell line, MISK81-5, was established from a metastatic lymph node of oral squamous cell carcinoma. Immunocytochemical and ultrastructural observations revealed an obvious epithelial origin of the cell line. Chromosome analysis revealed a hypertriploid karyotype with numerical and structural anomalies. MISK81-5 cells could form a tumor mass in the subcutaneous tissue of recipient BALB/c athymic mice only when coinjected with Matrigel. A stem cell assay revealed that conditioned medium (CM) of MISK81-5 contained granulocyte colony-stimulating factor (G-CSF) or interleukin-6 activity. Quantitation by ELISA disclosed a higher concentration of G-CSF in the CM of MISK81-5 than in the CM of other squamous and gastric carcinoma cell lines. The sMISK, that was derived from MISK81-5 as a subpopulation of the cell line having higher tumorigenicity, also showed a similar hematopoietic stimulating activity to that of MISK81-5. These characteristics of the MISK81-5 cell line and its subpopulation, sMISK will be useful for studying the biological behavior of oral squamous cell carcinomas and its relation to hematopoietic stimulating factors.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0910-5050
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1257-62
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7531680-Aged,
pubmed-meshheading:7531680-Aged, 80 and over,
pubmed-meshheading:7531680-Aneuploidy,
pubmed-meshheading:7531680-Animals,
pubmed-meshheading:7531680-Carcinoma, Squamous Cell,
pubmed-meshheading:7531680-Cocarcinogenesis,
pubmed-meshheading:7531680-Collagen,
pubmed-meshheading:7531680-Culture Media, Conditioned,
pubmed-meshheading:7531680-Drug Combinations,
pubmed-meshheading:7531680-Female,
pubmed-meshheading:7531680-Granulocyte Colony-Stimulating Factor,
pubmed-meshheading:7531680-Humans,
pubmed-meshheading:7531680-Interleukin-6,
pubmed-meshheading:7531680-Laminin,
pubmed-meshheading:7531680-Lymph Nodes,
pubmed-meshheading:7531680-Male,
pubmed-meshheading:7531680-Mice,
pubmed-meshheading:7531680-Mice, Inbred BALB C,
pubmed-meshheading:7531680-Mice, Nude,
pubmed-meshheading:7531680-Mouth Neoplasms,
pubmed-meshheading:7531680-Neoplasm Transplantation,
pubmed-meshheading:7531680-Proteoglycans,
pubmed-meshheading:7531680-Tumor Cells, Cultured
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pubmed:year |
1994
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pubmed:articleTitle |
New human oral squamous carcinoma cell line and its tumorigenic subline producing granulocyte colony-stimulating factor.
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pubmed:affiliation |
Department of Oral Pathology, Faculty of Dentistry, Kyushu University, Fukuoka.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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