|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1995-3-6
|
|
pubmed:abstractText |
The B cell activation molecule CD40 and the p55 tumor necrosis factor receptor (p55TNFR) belong to the same family of structurally conserved proteins. We constructed a chimeric receptor consisting of the CD40 extracellular and transmembrane domains and the p55TNFR intracellular domain. This receptor hybrid retained the biological activity and the ligand specificity of the respective wild-type receptor domains. Thus it exerted a marked cytotoxic effect in three different transfected cell lines after activation not only with anti-CD40 antibody but also with CD40 ligand (CD40L) in soluble and membrane-bound forms. Using hybrid-transfected baby hamster kidney cells we demonstrated that herpesvirus saimiri-transformed human CD4+ T lymphocytes constitutively express bioactive CD40 ligand on their surface. The hybrid receptor-based assay was highly specific for CD40 activating reagents and more sensitive than an assay measuring CD40-mediated B cell rescue from apoptosis. Hence CD40/p55TNFR transfectants may be useful for dissecting CD40L-mediated events in T-B cell interactions, and also to detect a defective CD40L molecule in putative hyper-IgM syndrome patients.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD40,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/CD40 Ligand,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Tumor Necrosis Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0014-2980
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
25
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
80-6
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:7531151-Animals,
pubmed-meshheading:7531151-Antibodies, Monoclonal,
pubmed-meshheading:7531151-Antigens, CD,
pubmed-meshheading:7531151-Antigens, CD40,
pubmed-meshheading:7531151-Antigens, Differentiation, B-Lymphocyte,
pubmed-meshheading:7531151-Base Sequence,
pubmed-meshheading:7531151-CD40 Ligand,
pubmed-meshheading:7531151-Cell Line, Transformed,
pubmed-meshheading:7531151-Cricetinae,
pubmed-meshheading:7531151-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:7531151-Herpesvirus 2, Saimiriine,
pubmed-meshheading:7531151-Humans,
pubmed-meshheading:7531151-Membrane Glycoproteins,
pubmed-meshheading:7531151-Mice,
pubmed-meshheading:7531151-Molecular Sequence Data,
pubmed-meshheading:7531151-Receptors, Interleukin,
pubmed-meshheading:7531151-Receptors, Interleukin-4,
pubmed-meshheading:7531151-Receptors, Tumor Necrosis Factor,
pubmed-meshheading:7531151-Recombinant Fusion Proteins,
pubmed-meshheading:7531151-T-Lymphocytes,
pubmed-meshheading:7531151-Transfection
|
|
pubmed:year |
1995
|
|
pubmed:articleTitle |
A cytotoxic CD40/p55 tumor necrosis factor receptor hybrid detects CD40 ligand on herpesvirus saimiri-transformed T cells.
|
|
pubmed:affiliation |
Institute for Immunology, University of Munich, Germany.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
