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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1994-11-21
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pubmed:abstractText |
Human umbilical cord blood CD34+ cells were purified using a two-step procedure by elimination of the soybean agglutinin-binding cells and by a positive panning selection with a CD34 monoclonal antibody. The isolated fraction was 88-97% pure CD34+ cells. A yield of 48.5% was obtained when comparing the number of cells recovered in the CD34(+)-purified fraction and the number of CD34+ cells detected in the initial mononuclear cell fraction. By flow cytometry, we observed that the CD34+ cells that were not recovered were those that had the lower expression of CD34 antigen and were therefore the more mature cells. A high recovery of CFU-GEMM progenitors (73.9%) was also observed. These data suggest the possibility of purifying CD34+ umbilical cord blood cells for clinical applications, in particular for umbilical cord blood banking.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34,
http://linkedlifedata.com/resource/pubmed/chemical/Hematopoietic Cell Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Soybean Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/soybean lectin
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pubmed:status |
MEDLINE
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pubmed:issn |
1061-6128
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
275-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7522882-Antibodies, Monoclonal,
pubmed-meshheading:7522882-Antigens, CD,
pubmed-meshheading:7522882-Antigens, CD34,
pubmed-meshheading:7522882-Cell Count,
pubmed-meshheading:7522882-Cell Separation,
pubmed-meshheading:7522882-Colony-Forming Units Assay,
pubmed-meshheading:7522882-Feasibility Studies,
pubmed-meshheading:7522882-Fetal Blood,
pubmed-meshheading:7522882-Flow Cytometry,
pubmed-meshheading:7522882-Hematopoietic Cell Growth Factors,
pubmed-meshheading:7522882-Hematopoietic Stem Cells,
pubmed-meshheading:7522882-Humans,
pubmed-meshheading:7522882-Immunosorbent Techniques,
pubmed-meshheading:7522882-Infant, Newborn,
pubmed-meshheading:7522882-Lectins,
pubmed-meshheading:7522882-Plant Lectins,
pubmed-meshheading:7522882-Recombinant Proteins,
pubmed-meshheading:7522882-Soybean Proteins
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pubmed:year |
1993
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pubmed:articleTitle |
Human umbilical cord blood CD34+ cell purification with high yield of early progenitors.
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pubmed:affiliation |
Laboratoire de Biologie Cellulaire et Moléculaire des Facteurs de Croissance, Centre National de la Recherche Scientifique UPR 272, Villejuif, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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