Linked Life Data

7522206

Source:http://linkedlifedata.com/resource/pubmed/id/7522206

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1994-10-19
pubmed:abstractText
We determined the activity of phosphofructokinase (PFK), a rate-limiting enzyme of glycolysis, in the rat oocyte during maturation either by luteinizing hormone stimulation in vivo or by cultivation under various conditions. In vivo, the activity of PFK in the maturing oocyte increased significantly, suggesting that the glycolytic pathway developed as the first meiotic division proceeded. A breakdown of the germinal vesicle and an increased activity of PFK were observed when denuded oocytes were cultured. Treatment of the oocytes with dibutyryl cAMP and 3-isobutyl-1-methyl xanthine almost completely inhibited germinal breakdown, whereas the activity of PFK increased significantly. The data suggest that regulation of PFK activity is not directly dependent on cAMP or cAMP-dependent protein kinase, and is not necessarily dependent on meiotic maturation. Our study also revealed that epidermal growth factor (EGF) significantly increased PFK activity, showing that EGF may be a potent inducer of oocyte maturation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0301-0163
pubmed:author
pubmed:issnType
Print
pubmed:volume
41 Suppl 1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
63-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Phosphofructokinase activity as a measure of maturation of rat oocytes developed in vivo and in vitro.
pubmed:affiliation
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't