Linked Life Data

7520800

Source:http://linkedlifedata.com/resource/pubmed/id/7520800

Statements in which the resource exists.
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pubmed-article:7520800pubmed:abstractTextA cDNA clone containing a 921 bp open-reading frame (307 amino acids; 34 kDa) homologous to the TATA-binding protein (TBP) was isolated and sequenced from a Spodoptera frugiperda cell line that is commonly used in the baculovirus expression system. Analysis of the S. frugiperda TBP (SfTBP) sequence showed that the amino-terminal portion of SfTBP diverged significantly from that of other TBP sequences including Drosophila melanogaster whereas the carboxy-terminal sequence was highly conserved. Southern blot analysis indicated that SfTBP was encoded by a single gene in the S. frugiperda genome. Northern blot analysis indicated that steady-state levels of the 1.3 kb SfTBP transcript declined by 24 h post-infection corresponding to the time of virus-induced inhibition of host-cell transcription. Corresponding western blot analysis showed that TBP protein levels remain constant up to 72 h post-infection.lld:pubmed
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pubmed-article:7520800pubmed:articleTitleCharacterization of the Spodoptera frugiperda TATA-binding protein: nucleotide sequence and response to baculovirus infection.lld:pubmed
pubmed-article:7520800pubmed:affiliationDepartment of Agricultural Chemistry, Oregon State University, Corvallis 97331-7301.lld:pubmed
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pubmed-article:7520800pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:7520800pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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