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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1994-9-29
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pubmed:databankReference |
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pubmed:abstractText |
A cDNA clone containing a 921 bp open-reading frame (307 amino acids; 34 kDa) homologous to the TATA-binding protein (TBP) was isolated and sequenced from a Spodoptera frugiperda cell line that is commonly used in the baculovirus expression system. Analysis of the S. frugiperda TBP (SfTBP) sequence showed that the amino-terminal portion of SfTBP diverged significantly from that of other TBP sequences including Drosophila melanogaster whereas the carboxy-terminal sequence was highly conserved. Southern blot analysis indicated that SfTBP was encoded by a single gene in the S. frugiperda genome. Northern blot analysis indicated that steady-state levels of the 1.3 kb SfTBP transcript declined by 24 h post-infection corresponding to the time of virus-induced inhibition of host-cell transcription. Corresponding western blot analysis showed that TBP protein levels remain constant up to 72 h post-infection.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0965-1748
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
699-708
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:7520800-Amino Acid Sequence,
pubmed-meshheading:7520800-Animals,
pubmed-meshheading:7520800-Baculoviridae,
pubmed-meshheading:7520800-Base Sequence,
pubmed-meshheading:7520800-Blotting, Northern,
pubmed-meshheading:7520800-Cell Line,
pubmed-meshheading:7520800-Cloning, Molecular,
pubmed-meshheading:7520800-DNA Primers,
pubmed-meshheading:7520800-DNA-Binding Proteins,
pubmed-meshheading:7520800-Drosophila melanogaster,
pubmed-meshheading:7520800-Genetic Variation,
pubmed-meshheading:7520800-Genetic Vectors,
pubmed-meshheading:7520800-Molecular Sequence Data,
pubmed-meshheading:7520800-Moths,
pubmed-meshheading:7520800-Open Reading Frames,
pubmed-meshheading:7520800-Phylogeny,
pubmed-meshheading:7520800-Polymerase Chain Reaction,
pubmed-meshheading:7520800-RNA,
pubmed-meshheading:7520800-Sequence Homology, Amino Acid,
pubmed-meshheading:7520800-TATA Box,
pubmed-meshheading:7520800-TATA-Box Binding Protein,
pubmed-meshheading:7520800-Transcription, Genetic,
pubmed-meshheading:7520800-Transcription Factors
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pubmed:year |
1994
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pubmed:articleTitle |
Characterization of the Spodoptera frugiperda TATA-binding protein: nucleotide sequence and response to baculovirus infection.
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pubmed:affiliation |
Department of Agricultural Chemistry, Oregon State University, Corvallis 97331-7301.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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