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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1996-1-17
|
| pubmed:abstractText |
We have examined the temporal and spatial distribution of myofibroblast-like cells, a phenotype with fibroblast and smooth muscle features, in an experimental model of renal infection. Escherichia coli organisms (10(5)) were inoculated directly into the renal cortex of Sprague-Dawley rats weighing 270 g. Saline was substituted in a control group. The animals were sacrificed at five time points up to day 24 (E. coli n = 8, controls n = 3 each interval). Myofibroblasts were identified by morphology and immunohistochemistry for alpha smooth muscle actin (alpha-SMA) and compared with staining for monocytes (ED-1), collagen III, and bromodeoxyuridine incorporation. Histological changes included a focal lesion in E. coli infected animals. Interstitial alpha-SMA staining was confined to spindle-shaped cells resembling myofibroblasts. The percent fractional area of alpha-SMA staining in the lesion increased from 0.12 +/- 0.09 at day 1 to 20.0 +/- 7.1 at day 3 (p < 0.005), decreasing progressively to 2.0 +/- 2.6 by day 24. This paralleled bromodeoxyuridine incorporation in myofibroblasts: 0.4 +/- 0.5 cells/0.25 mm2 at day 1, 105.0 +/- 36.3 at day 3, and 2.6 +/- 2.2 cells/0.25 mm2 at day 24. ED-1-positive cells increased from 374 +/- 200/0.25 mm2 at day 1 to 894 +/- 88 at day 3 (p < 0.01), declining to 230 +/- 108/0.25 mm2 by day 24. Intracellular collagen III and alpha-SMA stainings were colocalized at day 3. The fractional area of collagen III increased by day 24 (p < 0.05). In conclusion, myofibroblasts accumulate transiently during renal interstitial fibrosis and are derived at least in part from local proliferation.(ABSTRACT TRUNCATED AT 250 WORDS)
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0250-8095
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
15
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
411-7
|
| pubmed:dateRevised |
2007-2-14
|
| pubmed:meshHeading |
pubmed-meshheading:7503141-Actins,
pubmed-meshheading:7503141-Analysis of Variance,
pubmed-meshheading:7503141-Animals,
pubmed-meshheading:7503141-Cell Division,
pubmed-meshheading:7503141-Cicatrix,
pubmed-meshheading:7503141-Collagen,
pubmed-meshheading:7503141-Escherichia coli Infections,
pubmed-meshheading:7503141-Female,
pubmed-meshheading:7503141-Fibroblasts,
pubmed-meshheading:7503141-Immunohistochemistry,
pubmed-meshheading:7503141-Kidney,
pubmed-meshheading:7503141-Kidney Diseases,
pubmed-meshheading:7503141-Muscle, Smooth,
pubmed-meshheading:7503141-Rats,
pubmed-meshheading:7503141-Rats, Sprague-Dawley
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Interstitial myofibroblasts in experimental renal infection and scarring.
|
| pubmed:affiliation |
Department of Nephrology, Royal Melbourne Hospital, Vic., Australia.
|
| pubmed:publicationType |
Journal Article
|