Linked Life Data

7498429

Source:http://linkedlifedata.com/resource/pubmed/id/7498429

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1996-1-18
pubmed:abstractText
Salivary gland extracts from the yellow fever mosquito Aedes aegypti contain a factor Xa-specific anticoagulant detectable in vitro by clotting assays. The anticoagulant is found only in female salivary glands, which is consistent with its role in bloodfeeding. Extracts of adult female glands significantly delay both the prothrombin time and the activated partial thromboplastin time, but have no effect on the thrombin clotting time. The anticoagulant has been shown to result from the specific inhibition of coagulation factor Xa based on a FXa clotting assay and the inhibition of FXa-directed cleavage of the synthetic chromogenic substrate, chromozym X. The inhibition of FXa by female salivary gland extracts exhibits noncovalent, noncompetitive inhibition kinetics and is reversible. The anticoagulant is destroyed by boiling for 10 min or heating at 56 degrees C for 30 min, has a 4 mM calcium optimum with no magnesium requirement, and has a pH optimum of 8.0. The anticoagulant activity has an apparent molecular weight of 35.5 kDa, as determined by molecular sieve. We propose that the A. aegypti anticoagulant is a novel, proteinaceous serine protease inhibitor specific for FXa.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0014-4894
pubmed:author
pubmed:issnType
Print
pubmed:volume
81
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
321-31
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
A factor Xa-directed anticoagulant from the salivary glands of the yellow fever mosquito Aedes aegypti.
pubmed:affiliation
Department of Molecular Biology and Biochemistry, University of California, Irvine 92717, USA.
pubmed:publicationType
Journal Article