Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1996-1-2
pubmed:abstractText
Type II integral membrane proteins are anchored by a signal-peptide/membrane-anchor domain (SA domain) located near their N-terminus, whereas type I membrane proteins are anchored by stop-transfer sequences usually located near the C-terminus. In this study we have attempted to transform neutral endopeptidase-24.11 (EC 3.4.24.11; NEP), a type II membrane protein, into a type I membrane protein. Three type I mutant proteins were constructed by fusion of topogenic sequences to the C-terminus of SecNEP, a soluble form of NEP. The first two type I mutants, SecNEP-TMC and SecNEP-TMIC, were constructed by fusing in frame the cytosolic and SA domains of NEP to the C-terminus of SecNEP. These two fusion proteins differ only in the orientation of the cytosolic tail. The third type I mutant, SecNEP-ACE, was constructed by fusing in frame the stop-transfer and cytosolic domains of angiotensin I-converting enzyme (EC 3.4.15.1; ACE) to the C-terminus of SecNEP. Our results suggest that: (1) the NEP ectodomain can be anchored with a type I topology in the endoplasmic reticulum (ER) membrane by both NEP and ACE topogenic sequences; (2) SecNEP-TMC and SecNEP-TMIC were transport-incompetent and needed proteolytic cleavage in the C-terminal region to leave the ER, whereas SecNEP-ACE was transported out of the ER as a type I membrane protein. Therefore we concluded that the nature of topogenic sequences determines the transport-competence of topological mutants of neutral endopeptidase-24.11.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-1419038, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-1682055, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-1729684, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-1733974, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-1826760, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-1848554, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-1918086, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-1985894, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-1997206, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-2197415, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-2275815, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-2275818, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-2334403, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-2440677, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-2458768, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-2670943, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-2688707, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-2762295, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-2808416, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-2824483, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-2838906, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-2839492, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-2849100, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-2894375, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-2993864, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-3001650, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-3027102, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-3294860, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-3474623, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-3791411, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-3907854, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-6929499, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-7702590, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-7919780, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-8103052, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-8181060, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-8198548, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-8253769, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-8253791, http://linkedlifedata.com/resource/pubmed/commentcorrection/7492341-8433362
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
312 ( Pt 1)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
99-105
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:7492341-Amino Acid Sequence, pubmed-meshheading:7492341-Animals, pubmed-meshheading:7492341-Biological Transport, pubmed-meshheading:7492341-Cell Line, pubmed-meshheading:7492341-Cell Membrane, pubmed-meshheading:7492341-Endoplasmic Reticulum, pubmed-meshheading:7492341-Glycoside Hydrolases, pubmed-meshheading:7492341-Immunoblotting, pubmed-meshheading:7492341-Kinetics, pubmed-meshheading:7492341-Membrane Proteins, pubmed-meshheading:7492341-Molecular Sequence Data, pubmed-meshheading:7492341-Mutagenesis, Site-Directed, pubmed-meshheading:7492341-Neprilysin, pubmed-meshheading:7492341-Peptidyl-Dipeptidase A, pubmed-meshheading:7492341-Rabbits, pubmed-meshheading:7492341-Recombinant Fusion Proteins, pubmed-meshheading:7492341-Transfection, pubmed-meshheading:7492341-beta-N-Acetylhexosaminidases
pubmed:year
1995
pubmed:articleTitle
The nature of topogenic sequences determines the transport competence of topological mutants of neutral endopeptidase-24.11.
pubmed:affiliation
Département de biochimie, Faculté de médecine, Université de Montréal, Quebec, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't