Linked Life Data

7408871

Source:http://linkedlifedata.com/resource/pubmed/id/7408871

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1980-11-25
pubmed:abstractText
1. The anaerobic microorganism Eubacterium limosum (DSM 20 402), grown in the presence of L-[methyl-14C]methionine, produces vitamin B12 not only labeled in the corrin ring, but also in the 5,6-dimethylbenzimidazole moiety. The vitamin B12 obtained in a similar experiment with L-[methyl-13C]methionine showed nine signals in the 13CV NMR spectrum. Seven of these signals are due to the seven 'extra' methyl groups of the corrin ring, two signals originate from the two methyl groups of the 5,6-dimethylbenzimidazole moiety. This result is in contrast to previous experiments with aerobic and aerotolerant organisms, where L-methionine is only the precursor of the seven corrin methyl groups, and the 5,6-dimethylbenzimidazole is formed from riboflavin. 2. E. limosum transforms exogenously added benzimidazole into benzimidazolylcobamide, but with [2-14C]benzimidazole it was shown that benzimidazole is not a precursor of the 5,6-dimethylbenzimidazole moiety of vitamin B12. 3. Radioactivity from [2-14C]glycine is almost exclusively incorporated into the 5,6-dimethylbenzimidazole moiety of vitamin B12, but not into the corrin moiety. This demonstrates that in E. limosum delta-aminolevulinic acid, the precursor of the corrin moiety, is not formed as in many other organisms from succinyl-CoA and glycine.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:volume
109
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
115-8
pubmed:dateRevised
2007-7-23
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Biosynthesis of vitamin B12. Experiments with the anaerobe Eubacterium limosum and some labelled substrates.
pubmed:publicationType
Journal Article