Choline stimulated secretion of catecholamines from primary dissociated cultures of bovine adrenal medullary chromaffin cells by interacting with nicotinic receptors. Secretion was readily detected at a choline concentration of 1 millimole per liter and was maximal at 3 to 10 millimoles per liter; it was completely calcium-dependent. Further analysis suggested that choline acts as a partial nicotinic agonist.
