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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
9
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pubmed:dateCreated |
1981-10-25
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pubmed:abstractText |
We described an assay for theophylline in serum in which the reagents for the substrate-labeled fluorescent immunoassay procedure are dry reagents in a paper matrix. This assay format is both rapid and convenient. In one such paper strip assay, the competing drug conjugate is present in the diluting buffer (dilution-into-conjugate strip assay); in another, all necessary assay reagents are contained on the strip (integral strip assay). The latter strip can be prepared by using appropriate solvents in a "two-dip" procedure, which prevents premature reaction of the assay constituents. The dilution-into-conjugates strip assays yield results that compare well with those by the corresponding solution assay (r = 0.975, standard error of estimate = 1.67 mg/L) and by "high-performance" liquid chromatography (r = 0.980, standard error of estimate = 1.49 mg/L). The integral strip results were compared with those by an enzyme immunoassay for theophylline (r = 0.990, standard error of estimate = 2.07 mg/L). Both strip assays require only a single dilution step before analysis.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0009-9147
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1614-7
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:7261339-Humans,
pubmed-meshheading:7261339-Immunoassay,
pubmed-meshheading:7261339-Indicators and Reagents,
pubmed-meshheading:7261339-Kinetics,
pubmed-meshheading:7261339-Reagent Strips,
pubmed-meshheading:7261339-Spectrometry, Fluorescence,
pubmed-meshheading:7261339-Theophylline
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pubmed:year |
1981
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pubmed:articleTitle |
Homogeneous fluorescent immunoassay with dry reagents.
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pubmed:publicationType |
Journal Article
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