Linked Life Data

7240650

Source:http://linkedlifedata.com/resource/pubmed/id/7240650

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
1981-8-10
pubmed:abstractText
A method for quantitative estimation of glycerol and total carbohydrate in biological samples is described. The samples, deproteinized with cold acetone or trichloroacetic acid, were treated in the cold with 10 vols. of 0.75% (w/w) anthrone in 84% (w/w) sulphuric acid, and then heated 10 min at 100 degrees C. Absorbance at 590 nm was used for evaluation of total carbohydrate content in the sample. The absorbance at 510 nm was used for the combined carbohydrate and glycerol estimation. This latter observation leads to the determination of the glycerol content of the sample because the carbohydrate interference is known from the data obtained at 590 nm. Uses of this method to determine glycerol and carbohydrate content in lipids of chicken egg-yolk samples are presented.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0165-022X
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
227-31
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
A method for the simultaneous determination of total carbohydrate and glycerol in biological samples with the anthrone reagent.
pubmed:publicationType
Journal Article