Linked Life Data

7204485

Source:http://linkedlifedata.com/resource/pubmed/id/7204485

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-5-13
pubmed:abstractText
Biochemical evidence from the preceding paper indicated that [3H]N-acetylmannosamine may be used as a fairly specific precursor for the sialic acid residues of glycoproteins (and perhaps glycolipids) in radioautographs of rat liver and duodenum. In order to study the site of incorporation of this label in cell types of various tissues, we gave 40-g rats and 15-g Swiss albino mice a single intravenous injection of 8 mCi of [3H]N-acetylmannosamine and sacrificed them after 2 and 10 min. To trace the subsequent migration of the labeled glycoproteins, we injected 40-g rats with 4 mCi of [3H]N-acetylmannosamine and sacrificed them after 20 and 30 min, 1, 4, and 24 h, and 3 and 9 d. Light microscope radioautographic analysis revealed that in a great variety of cell types the label was initially localized to the Golgi region. Electron microscope radioautographic analysis of duodenal villous columnar and goblet cells, pancreatic acinar cells and Paneth cells, from rats and mice sacrificed 10 min after injection, showed that the silver grains were localized over Golgi saccules (and adjacent secretion granules). In kidney proximal and distal tubule cells reaction was initially localized to the Golgi apparatus in some areas of the kidney cortex whereas in other areas it was more diffuse. In all cells, the proportion of silver grains over the Golgi apparatus decreased with time after injection while an increasing number of grains appeared over secretion products in secretory cells or over the plasma membrane in other cell types. Lysosomes also became increasingly labeled at later time intervals. The above results suggest that in most cell types sialic acid residues are incorporated into glycoproteins (and perhaps glycolipids), primarily in the Golgi apparatus. With time, these newly synthesized molecules migrate to secretion products, to the plasma membrane, or to the lysosomes.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7204485-1031913, http://linkedlifedata.com/resource/pubmed/commentcorrection/7204485-185634, http://linkedlifedata.com/resource/pubmed/commentcorrection/7204485-198392, http://linkedlifedata.com/resource/pubmed/commentcorrection/7204485-410770, http://linkedlifedata.com/resource/pubmed/commentcorrection/7204485-4272599, http://linkedlifedata.com/resource/pubmed/commentcorrection/7204485-43006, http://linkedlifedata.com/resource/pubmed/commentcorrection/7204485-4331504, http://linkedlifedata.com/resource/pubmed/commentcorrection/7204485-4809242, http://linkedlifedata.com/resource/pubmed/commentcorrection/7204485-5449186, http://linkedlifedata.com/resource/pubmed/commentcorrection/7204485-5966171, http://linkedlifedata.com/resource/pubmed/commentcorrection/7204485-7204482, http://linkedlifedata.com/resource/pubmed/commentcorrection/7204485-848452, http://linkedlifedata.com/resource/pubmed/commentcorrection/7204485-865081, http://linkedlifedata.com/resource/pubmed/commentcorrection/7204485-914649
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
16-28
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
The site of incorporation of sialic acid residues into glycoproteins and the subsequent fates of these molecules in various rat and mouse cell types as shown by radioautography after injection of [3H]N-acetylmannosamine. II. Observations in tissues other than liver.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't