Linked Life Data

7104701

Source:http://linkedlifedata.com/resource/pubmed/id/7104701

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1982-10-12
pubmed:abstractText
The endocytosis of [125I]nerve growth factor (NGF) by cultured rat pheochromocytoma cells, the PC12 line, was studied by ultrastructural quantitative autoradiography. Cells previously grown in the absence of NGF were incubated at 37 degrees C with [125I]NGF for periods of up to 26 h. Under these conditions, PC12 cells have not yet shown outgrowth of neurites. Heavy labeling of the plasma membrane was observed at 2 h. At 6 and 26 h, lower but significant levels of labeling of the plasma membrane were still noted. After 2, 6 and 26 h, endocytosis of [125I]NGF was detected. Low breakdown of [125I]NGF was observed only after 26 h. At 26 h, grain density distributions of [125I]NGF showed significant labeling of lysosomes, while nuclei and rough endoplasmic reticulum showed the lowest levels of labeling. Significant apparent labeling of vesicles of smooth endoplasmic reticulum and of various cytoplasmic components, including cytoskeletal elements, was also observed. These findings indicate that [125I]NGF undergoes endocytosis quite slowly. During the initial phase of the interaction between NGF and PC12 cells, plasma membrane moieties are constantly labeled while lysosomes show progressively increasing uptake of NGF. The pathway of endocytosis of [125I]NGF included vesicles of the smooth endoplasmic reticulum but the Golgi apparatus was not unequivocally labeled. The findings do not support the hypothesis that NGF is transported to the nucleus for the initiation of transcriptional events. Our morphologic observations are consistent with the hypothesis that NGF constantly occupies sites on or adjacent to the plasma membrane, and that it slowly undergoes endocytosis into lysosomes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
241
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
145-56
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Endocytosis of nerve growth factor by PC12 cells studied by quantitative ultrastructural autoradiography.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't