Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1982-7-8
pubmed:abstractText
In this study, we have examined the profiles of proteins synthesized by mammalian oocytes in the presence and absence of cumulus cells. The results show that the patterns of protein synthesis are broadly similar in cumulus-enclosed and denuded oocytes, but that as a consequence of removing the cumulus cells, the presence of a 45,000-dalton band is greatly reduced. This band, identified by comigration studies on two-dimensional polyacrylamide gels as actin, is strongly synthesized in both isolated cumulus cells and in oocytes labeled in the presence of cumulus cells, but is only weakly synthesized by oocytes incubated in the absence of cumulus cells. We suggest that the presence of newly synthesized actin in the oocyte is dependent upon cellular cooperation between the cumulus cells and oocytes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-104X
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
220
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
125-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Cell interactions and actin synthesis in mammalian oocytes.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't