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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1981-9-25
|
| pubmed:abstractText |
A radioreceptor assay for insulin-like growth factor II (IGF-II) is described in which [125I]IGF-II is bound by rat placental membranes. IGF-I and other insulin-related peptides have less than 1% of the potency of IGF-II in displacing [125I]-IGF-II. IGF-II is extracted from its serum binding protein with a simple acid-ethanol step. After neutralization with Tris base, the extract is introduced directly into the radioreceptor assay. Results are expressed in terms of a reference serum with an assigned potency of 1 U/ml. By this method, serum from normal adults, 20-69 yr of age, had a mean (+/-SE)IGF-II activity of 0.73 +/- 0.03; higher concentrations were noted in adults more than 70 years of age (1.05 +/- 0.05), in cord serum (1.55 +/- 0.24), and in short children with normal GH secretion (0.88 +/- 0.42). In hypopituitary dwarfism, serum IGF-II activity was reduced (0.50 +/- 0.05), but in acromegaly, it was not increased (0.77 +/- 0.06). In 10 of 14 serum samples from patients with tumor-related hypoglycemia, the IGF-II exceeded the normal 95.5% confidence limits. In 8 of these sera, IGF-I by RIA was low, and in 5, it was essentially unmeasurable. These results with a new radioreceptor assay for IGF-II provide additional evidence that the regulation of this serum peptide differs from that of IGF-I.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0021-972X
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
53
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
289-94
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:7019228-Acromegaly,
pubmed-meshheading:7019228-Adult,
pubmed-meshheading:7019228-Aged,
pubmed-meshheading:7019228-Animals,
pubmed-meshheading:7019228-Child,
pubmed-meshheading:7019228-Dwarfism, Pituitary,
pubmed-meshheading:7019228-Female,
pubmed-meshheading:7019228-Fetal Blood,
pubmed-meshheading:7019228-Growth Hormone,
pubmed-meshheading:7019228-Humans,
pubmed-meshheading:7019228-Hypoglycemia,
pubmed-meshheading:7019228-Insulin,
pubmed-meshheading:7019228-Middle Aged,
pubmed-meshheading:7019228-Neoplasms,
pubmed-meshheading:7019228-Peptides,
pubmed-meshheading:7019228-Pregnancy,
pubmed-meshheading:7019228-Radioligand Assay,
pubmed-meshheading:7019228-Rats,
pubmed-meshheading:7019228-Somatomedins
|
| pubmed:year |
1981
|
| pubmed:articleTitle |
Measurement of insulin-like growth factor II by a specific radioreceptor assay in serum of normal individuals, patients with abnormal growth hormone secretion, and patients with tumor-associated hypoglycemia.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|