Linked Life Data

6986376

Source:http://linkedlifedata.com/resource/pubmed/id/6986376

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1980-4-23
pubmed:abstractText
The mechanism of streptolydigin inhibition of RNA synthesis has been investigated with a combination of steady state kinetics and product analysis by employing the abortive initiation reaction of Escherichia coli RNA polymerase. The pattern of inhibition by streptolydigin on the poly[d(A-T)] . poly[d(A-T)]template (non-competitive versus AMP; competitive versus UTP) was consistent with one inhibitor binding site and with an ordered addition of AMP followed by UTP. The more complicated patterns observed on the poly[d(I-C)] . poly[d(I-C)] template and the bacteriophage T7 A2 promotor (noncompetitive versus CTP) were explained by assuming that streptolydigin could stabilize the translocated ternary complex containing the product dinucleotide. Product analysis of two other abortive initiation reactions showed that the product did not dissociate from the inhibitor-bound translocated ternary complex. Finally, rifampicin and streptolydigin were shown to be functionally independent during initiation on several templates.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
255
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1610-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
On the mechanism of streptolydigin inhibition of Escherichia coli RNA polymerase.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.