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rdf:type |
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lifeskim:mentions |
umls-concept:C0005052,
umls-concept:C0010453,
umls-concept:C0013935,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0032961,
umls-concept:C0037211,
umls-concept:C0314603,
umls-concept:C1524026,
umls-concept:C1705241,
umls-concept:C1705242
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pubmed:issue |
6
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pubmed:dateCreated |
1980-11-25
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pubmed:abstractText |
Mouse embryos explanted at 7 1/2 or 8 1/2 days of gestation were cultured in medium containing benzo[a]pyrene and supplemented with 5-bromodeoxyuridine to allow detection of sister chromatid exchanges. The murine Ah locus regulates the inducible metabolism of polycyclic hydrocarbons such as benzo[a]pyrene. A high frequency of sister chromatid exchange was induced by benzo[a]pyrene in embryos from three Ah-"responsive" inbred strains (BALB/cDub, C3H/AnfCum, and C57BL/6N); there was little or no increase in two Ah-"nonresponsive" inbred strains (AKR/J and DBA/2J). Benzo[a]pyrene also induced sister chromatid exchanges in the Ah-responsive recombinant inbred line B6NXAKN-12 but not in the Ah-nonresponsive recombinant inbred line B6NXAKN-3. Sister chromatid exchange in cultured Ah-responsive mouse embryos was thus shown to be a sensitive assay. These data provide direct evidence that genetically responsive mouse embryos (early postimplantation stage) possess the subcellular processes necessary for induction of enzymes that metabolize benzo[a]pyrene to its chemically active forms(s). Both the Ah regulatory gene product (a cytoslic receptor) and the structural gene product (inducible cytochrome P1-450) therefore appear to be functional at an early embryonic age. Furthermore, this metabolic capacity may play an important role in the damage to embryonic cells by polycyclic hydracarbons.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-1101891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-14497634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-327020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-378536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-406414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-412268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-4135474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-4137928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-4138930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-4473724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-4502804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-4726445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-4910934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-4981257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-500663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-5055540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-5157583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-53133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-542892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-5484816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-5558564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-566850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-5723465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-5892565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-593288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-6100719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-631860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-74232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-768755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-77756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-817799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-852872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-854023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-955403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-958372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6932035-999784
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
77
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3524-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6932035-Animals,
pubmed-meshheading:6932035-Benzoflavones,
pubmed-meshheading:6932035-Benzopyrenes,
pubmed-meshheading:6932035-Biotransformation,
pubmed-meshheading:6932035-Cytochrome P-450 Enzyme System,
pubmed-meshheading:6932035-Embryo, Mammalian,
pubmed-meshheading:6932035-Enzyme Induction,
pubmed-meshheading:6932035-Female,
pubmed-meshheading:6932035-Male,
pubmed-meshheading:6932035-Maternal-Fetal Exchange,
pubmed-meshheading:6932035-Mice,
pubmed-meshheading:6932035-Mice, Inbred Strains,
pubmed-meshheading:6932035-Organ Culture Techniques,
pubmed-meshheading:6932035-Pregnancy,
pubmed-meshheading:6932035-Receptors, Drug,
pubmed-meshheading:6932035-Sister Chromatid Exchange,
pubmed-meshheading:6932035-Species Specificity,
pubmed-meshheading:6932035-beta-Naphthoflavone
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pubmed:year |
1980
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pubmed:articleTitle |
Cultured mouse embryos metabolize benzo[a]pyrene during early gestation: genetic differences detectable by sister chromatid exchange.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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