Linked Life Data

6896652

Source:http://linkedlifedata.com/resource/pubmed/id/6896652

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
1982-10-21
pubmed:abstractText
RNA-dependent RNA polymerases have been isolated in almost pure form from cucumber mosaic virus (CMV) infected cucumber cotyledons and from tobacco leaves and were compared with the less pure enzyme from uninfected tobacco. The purified polymerase from cucumber shows on sodium dodecyl sulfate gels two peptide chains of about 100 and 112 kdaltons. The enzyme from tobacco shows a close doublet of about 125 kdaltons, which is also present in the less pure preparation from healthy tobacco. While both the cucumber and tobacco enzymes can use many polynucleotides and RNAs as templates, considerable quantitative differences exist, poly(C) being by far the most effective template for the cucumber enzyme but of low activity with the tobacco enzyme and poly(UG) being highly active with the latter but not the former. Poly(A) and poly(G) are inactive. Different viral RNAs, including CMV RNA, show smaller differences. The sedimentation rates of the enzyme from both sources are the same as that of gamma-globulin. A uridine 5'-triphosphate (UTP) terminal transferase also present in both plants sediments much more slowly and can be completely removed from the RNA polymerases. However, slight nucleolytic activity remains associated with the purified polymerases and appears to be proportional to the polymerase activity. The conclusion derived from these data is that the RNA-dependent RNA polymerases of different plants differ and are not detectably affected by virus infection in qualitative terms while being produced in greatly increased amounts upon some virus infections. Similar conclusions were previously reached with less purified enzyme preparations from tobacco as compared to cowpea, infected with different viruses, if any.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3161-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Comparative studies on ribonucleic acid dependent RNA polymerases in cucumber mosaic virus infected cucumber and tobacco and uninfected tobacco plants.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S.