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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1983-7-15
|
| pubmed:abstractText |
We have identified four forms of sea urchin RNA polymerase (Ia, Ib, II and III). Three of the forms co-elute on DEAE cellulose chromatography but separate on DEAE Sephadex chromatography. The separation of these three enzyme forms by DEAE Sephadex chromatography can be eliminated with non-ionic detergent. We also demonstrate that either form I or form III RNA polymerase loses its resistance to alpha-amanitin after DEAE chromatography. A procedure for the purification of combined form I and III RNA polymerase and the purification of RNA polymerase II is also presented.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0032-7484
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
13
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
21-39
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:6856583-Animals,
pubmed-meshheading:6856583-Cell Nucleus,
pubmed-meshheading:6856583-DNA-Directed RNA Polymerases,
pubmed-meshheading:6856583-RNA Polymerase I,
pubmed-meshheading:6856583-RNA Polymerase II,
pubmed-meshheading:6856583-RNA Polymerase III,
pubmed-meshheading:6856583-Sea Urchins
|
| pubmed:year |
1983
|
| pubmed:articleTitle |
Purification and characterization of the RNA polymerases of the sea urchin, Lytechinus variegatus.
|
| pubmed:publicationType |
Journal Article
|