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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1980-8-25
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pubmed:abstractText |
We have measured the capacity of Pseudomonas fluorescens to transport the glucose analog 2-deoxy-d-glucose and the amino acids l-alanine and alpha-aminoisobutyric acid under conditions in which the cells could generate (i) both a membrane proton motive force and high-energy phosphate compounds, (ii) a proton motive force but not high-energy phosphate compounds, and (iii) neither a proton motive force nor high-energy phosphate compounds. This was done by depleting cells of adenosine triphosphate stores by treatment with sodium arsenate and then suspending them in a phosphate-free medium, where they could generate a proton motive force but not phosphate bond energy, or in a phosphate-containing medium, where they could generate both a proton motive force and phosphate bond energy. Inclusion of the proton-conducting ionophore carbonyl cyanide-m-chlorophenyl hydrazone under either condition precluded the generation of both a proton motive force and phosphate bond energy. The amino acids l-alanine and alpha-aminoisobutyric acid were transported independently of phosphate bond energy and required only a proton motive force. 2-Deoxy-d-glucose was transported only under conditions in which phosphate bond energy could be generated. These results are consistent with the findings of others that Pseudomonas aeruginosa produces an inducible shock-sensitive glucose-binding protein and conform to the generalization that binding protein-associated transport systems are energized by phosphate bond energy.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6769909-1262305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6769909-127561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6769909-139135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6769909-139858,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6769909-140802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6769909-143919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6769909-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6769909-26404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6769909-375230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6769909-407216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6769909-4197191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6769909-4199012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6769909-4199013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6769909-4205195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6769909-4214229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6769909-4264299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6769909-4279250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6769909-4281777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6769909-4427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6769909-4626609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6769909-780342
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
142
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
755-62
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6769909-Adenosine Triphosphate,
pubmed-meshheading:6769909-Alanine,
pubmed-meshheading:6769909-Aminoisobutyric Acids,
pubmed-meshheading:6769909-Arsenates,
pubmed-meshheading:6769909-Biological Transport, Active,
pubmed-meshheading:6769909-Carbonyl Cyanide m-Chlorophenyl Hydrazone,
pubmed-meshheading:6769909-Deoxy Sugars,
pubmed-meshheading:6769909-Deoxyglucose,
pubmed-meshheading:6769909-Dicyclohexylcarbodiimide,
pubmed-meshheading:6769909-Energy Metabolism,
pubmed-meshheading:6769909-Pseudomonas fluorescens
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pubmed:year |
1980
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pubmed:articleTitle |
Energization of glucose transport by Pseudomonas fluorescens.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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