Linked Life Data

6744295

Source:http://linkedlifedata.com/resource/pubmed/id/6744295

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1984-9-19
pubmed:abstractText
We found that 9L-2 cells, a cell line derived from the in vivo 9L rat brain tumor model, are approximately 8-fold more resistant to the cytotoxic effect of 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) than are sensitive 9L cells. Treatment with BCNU induces sister chromatid exchanges in both lines, but to produce similar levels of exchanges, 9L-2 cells must be treated with a 14-fold higher concentration of BCNU. The extent of DNA methylation was the same in both cell lines after a 1-hr treatment with 100 microM methylnitrosourea. While the levels of the alkylation products N-7-methylguanine and N-3-methyladenine were similar in both lines, the level of O6-methylguanine was 20% lower in 9L-2 than in 9L cells, which implies that 9L-2 cells repair O6-alkylguanine derivatives more efficiently than do 9L cells. The number of DNA interstrand cross-links formed in 9L-2 cells after treatment with BCNU was approximately 50% of the number formed in 9L cells. These results suggest that the repair of O6-alkylguanine derivatives formed in BCNU-treated 9L-2 cells may be related to the reduced number of DNA interstrand cross-links formed and may have a role in the mechanism of cellular resistance of 9L-2 cells to BCNU. However, our results indicate that, in itself, the reduction in the number of DNA cross-links may not be sufficient to account entirely for the cellular resistance of 9L-2 cells to BCNU and suggest that additional mechanisms may be involved in cellular resistance of 9L-2 cells to BCNU treatment.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3763-7
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Reduced level of DNA cross-links and sister chromatid exchanges in 1,3-bis(2-chloroethyl)-1-nitrosourea-resistant rat brain tumor cells.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't