rdf:type |
|
lifeskim:mentions |
umls-concept:C0015683,
umls-concept:C0022646,
umls-concept:C0023884,
umls-concept:C0054320,
umls-concept:C0184661,
umls-concept:C0206415,
umls-concept:C0680730,
umls-concept:C1148554,
umls-concept:C1510438,
umls-concept:C1707455,
umls-concept:C1882687
|
pubmed:issue |
1
|
pubmed:dateCreated |
1984-7-20
|
pubmed:abstractText |
The malonyl-CoA assay was nonlinear at low malonyl-CoA concentrations when labeled acetyl-CoA was used as fatty acid synthase primer. Linearity was obtained with low concentrations of both fatty acid synthase and labeled acetyl-CoA, but then the assay was disturbed by the diluting effect of endogenous acetyl-CoA. The problems of nonlinearity and dilution of radioactivity by endogenous compounds were absent when labeled butyryl-CoA was used as primer. The levels of malonyl-CoA in rat heart, kidney, and liver were determined. The use of butyryl-CoA gave higher values of malonyl-CoA.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Apr
|
pubmed:issn |
0003-2697
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
138
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
107-11
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:6731835-Acetyl Coenzyme A,
pubmed-meshheading:6731835-Acyl Coenzyme A,
pubmed-meshheading:6731835-Animals,
pubmed-meshheading:6731835-Fatty Acid Synthetase Complex,
pubmed-meshheading:6731835-Kidney,
pubmed-meshheading:6731835-Liver,
pubmed-meshheading:6731835-Male,
pubmed-meshheading:6731835-Malonyl Coenzyme A,
pubmed-meshheading:6731835-Myocardium,
pubmed-meshheading:6731835-Rats,
pubmed-meshheading:6731835-Rats, Inbred Strains
|
pubmed:year |
1984
|
pubmed:articleTitle |
Determination of malonyl-coenzyme A in rat heart, kidney, and liver: a comparison between acetyl-coenzyme A and butyryl-coenzyme A as fatty acid synthase primers in the assay procedure.
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|