Linked Life Data

6683261

Source:http://linkedlifedata.com/resource/pubmed/id/6683261

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1983-8-11
pubmed:abstractText
Several strains of mycobacteria were cultivable from Mycobacterium leprae-infected human and armadillo tissues in a liquid medium containing three dimethyl analogs: dimethylketone, dimethylsulfoxide, and tetradecane [CH3 . (CH2)12 . CH3]. The medium contained KH2PO4, 7.0 g; Na2HPO4, 1.0 g; (NH4)2SO4, 2.0 g; MgSO4, 0.1 g; iron ammonium citrate, 0.1 g; DMSO, 10 ml; and acetone, 150 ml in distilled water ad one liter. Tetradecane 0.1 ml was added aseptically to each tube, containing 10 ml of the sterile medium. The media, inoculated with M. leprae were incubated at 38 degrees C and shaken vigorously twice weekly. Growth developed as a fine emulsion at the upper phase of the two-phase system. This was homogenized by mechanical shaking, permitting growth estimation by turbidity measurements. Microscopic examination showed unmistakably the slow but abundant multiplication of acid-fast rods. The logarithmic growth rate was measurable during two to three months, followed by a plateau. The strains are maintained in subcultures by regular transfer into the same medium at two- to three-month intervals. The cultures and subcultures do not grow on Löwenstein or in Dubos media, but in the foot pads of mice they produce a multiplication similar to that obtained following injection of host-grown M. leprae. The cultures are tentatively designated as Mycobacterium X. The relationship of Mycobacterium X to the pathology of leprosy is not clear.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0148-916X
pubmed:author
pubmed:issnType
Print
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
77-83
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
In vitro cultivation of Mycobacterium X from Mycobacterium leprae-infected tissues in acetone-dimethylsulfoxide-tetradecane medium.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't