Linked Life Data

6665299

Source:http://linkedlifedata.com/resource/pubmed/id/6665299

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1984-3-7
pubmed:abstractText
It has been suggested that heparin-induced increases in drug free fraction are largely artifactual as a result of continued in vitro activity of lipoprotein lipases after blood samples collection. The influence of different concentrations of protamine (1.0, 2.5, 5.0 and 10.0 mg/ml), an inhibitor of lipoprotein lipases, on lidocaine free fraction was studied before (control) and 10 min after heparinization (3000 IU) in 7 cardiac catheterized patients. Heparin increased the mean free fraction of lidocaine by 85% (p less than 0.001) and was associated with more than 2-fold increases in free fatty acids (FFAs). The presence of protamine at 2.5, 5.0 and 10.0 mg/ml diminished both the heparin-induced elevation of lidocaine free fraction (p less than 0.001) and FFAs (p less than 0.001). At a protamine concentration of 10.0 mg/ml, the FFAs remained higher than control value (p less than 0.05) while the free fraction was not different from control preheparin value. There were significant relationships (p less than 0.01) between log protamine concentration and the changes in both free fraction (r2 = 0.906) and FFAs (r2 = 0.931). The inhibitor also reduced (p less than 0.01) the lidocaine free fraction in control samples but these changes were not correlated with changes of FFAs. These results indicate that it is impossible, at this point, to abolish the artifactual effect of heparin without altering protein binding of lidocaine.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0034-5164
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
401-15
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Influence of protamine on heparin-induced increases of lidocaine free fraction.
pubmed:publicationType
Journal Article, In Vitro