Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-2-14
pubmed:abstractText
A new one-dimensional agarose gel electrophoresis method for the quantitation of glycosaminoglycans in biological samples has been described. In this procedure, concanavalin A, suspended in agarose gel, interacts with glycosaminoglycans such that rocket-like precipitin lines are formed. The area of the rocket is directly proportional to the glycosaminoglycan content of the sample. This procedure permits measurement of glycosaminoglycans in amounts as low as 0.5 nmol uronic acid equivalents with a coefficient of variation of only 8%. The described method has been applied to the determination of free heparan sulfate in plasma. This method can also be used to measure all high-charge glycosaminoglycans of biological interest.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
134
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
240-4
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Quantitative determination of individual glycosaminoglycans in plasma by concanavalin A rocket electrophoresis.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.