Linked Life Data

6530508

Source:http://linkedlifedata.com/resource/pubmed/id/6530508

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
1985-5-20
pubmed:abstractText
A method for the estimation of pyruvate radioactivity in complex biological samples is presented. After perchloric acid deproteinization, the samples were divided in two aliquots. All pyruvate was removed from the first aliquot with lactate dehydrogenase and NADH; the other was left undisturbed. Then all hydrophilic components of the sample were removed through an Amberlite XAD-7 column. The samples were treated with dinitrophenylhydrazine to obtain the corresponding hydrazones, which were retained in an Amberlite XAD-7 column and then were eluted with acetone and their radioactivity counted. The difference between both aliquots corresponded to the pyruvate radioactivity in the sample. No contamination by lactate, glycerol, glucose or amino acids was observed. The mean recovery of 5 determinations of pyruvate radioactivity was 91.3% +/- 1.0.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0165-022X
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
181-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
An enzyme specific method for estimation of pyruvic acid radioactivity in biological samples.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't