Linked Life Data

6384948

Source:http://linkedlifedata.com/resource/pubmed/id/6384948

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1984-11-9
pubmed:abstractText
This investigation has combined microangiography, immunocytochemistry, coupled with transmission and scanning electron microscopy to discuss the neuroanatomical interactions that occur in the brains of Brattleboro rats with diabetes insipidus, following stereotaxic placement of normal fetal hypothalamic fragments into the third cerebral ventricle. Following surgical placement of 17 day post-coitus hypothalamic fragments, host rats with chronic autosomal homozygous diabetes insipidus were killed and their brains were prepared for analysis. A significant degree of explants (68%) flourished and grew in the lumen of the third cerebral ventricle of recipient hosts. Explants were rapidly invaded by host vessels from two routes. Vessels arose from the underlying mantle plexus of portal capillaries which remained fenestrated in the lower one-third of the explants and developed neurovascular (neurohemal) zones. The second source of vessels arose from bed capillaries of the adjacent paraventricular nucleus and adjacent hypothalamus. In contrast to vessels arising from the contact zone, these latter vessels remained unfenestrated. Small clusters of immunocytochemically positive neurons (neurophysin positive) were seen throughout the explants. Numerous healthy magnocellular neurons harboring numerous dense core vesicles and exhibiting multiple axosomatic and axodendritic synapses were seen throughout the neuropil of explants. Axon profiles were noted to terminate upon the abluminal basal lamina of perivascular spaces surrounding fenestrated capillaries in the lower one third of explants. None of the host animals exhibited physiological return to normal parameters of urine output, drinking behavior, and/or urine osmolarity. However the growth and development of explants in the third cerebral ventricle of DI hosts coupled with the emergence of bonafide neurovascular zones supports a potential anatomical substrate for the central delivery of neuropeptide hormones in this experimental model.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0196-9781
pubmed:author
pubmed:issnType
Print
pubmed:volume
5 Suppl 1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
169-83
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
The neuroanatomical and neurovascular organization of normal fetal hypothalamic explants in the third cerebral ventricle of Brattleboro rats with homozygous diabetes insipidus.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.