Linked Life Data

6384728

Source:http://linkedlifedata.com/resource/pubmed/id/6384728

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-11-14
pubmed:abstractText
Three amber mutants of Escherichia coli, dnaG9, dnaG24 and dnaG26, affected in the structural gene (dnaG) for "primase" have been isolated from a parental strain carrying a temperature-sensitive amber suppressor (supF-Ts6). These mutants grow at 30 degrees C but not at 42 degrees C since primase is essential for growth and is synthesized only at low temperatures. Chimeric plasmids carrying dnaG+ but no other chromosomal genes of E. coli complemented the amber mutations, and the plasmid carrying a part of dnaG lost the complementing activity. Beside, plasmids carrying a dnaG amber mutation complemented a temperature-sensitive dnaG mutation only in the presence of amber suppressor. One of the amber mutations, dnaG24 which maps proximal to the NH2-terminus of the dnaG gene, exerted a polar effect on the synthesis of RNA polymerase sigma factor in E. coli.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0026-8925
pubmed:author
pubmed:issnType
Print
pubmed:volume
196
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
179-82
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Amber dnaG mutation exerting a polar effect on the synthesis of RNA polymerase sigma factor in Escherichia coli.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't