Linked Life Data

6384191

Source:http://linkedlifedata.com/resource/pubmed/id/6384191

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-11-15
pubmed:abstractText
Escherichia coli clones that express Legionella pneumophila antigens were isolated from a plasmid genomic library, and their antigens were characterized by immunoblotting with rabbit anti-L. pneumophila sera. Because previous studies of L. pneumophila antigens by whole-cell radioimmunoprecipitation suggested that comigrating native antigens were surface localized, we conducted experiments to determine if the cloned antigens were surface expressed in E. coli. Aliquots of antisera were absorbed by intact cells of three representative antigen-producing E. coli clones, and surface-bound antibodies were acid eluted from the intact cells. Immunoblots made with selectively absorbed antisera and eluted antibodies confirmed that reactivity to the homologous cloned antigens could be specifically absorbed from the antisera and then eluted from the cells, demonstrating a surface (antibody-accessible) localization in the cloned state. Antibodies eluted from the surface of an E. coli clone that expressed a 19-kilodalton antigen reacted with the surface of L. pneumophila in a liquid-phase, whole-cell enzyme-linked immunosorbent assay. In addition, intact cells of this clone were used in an enzyme-linked immunosorbent assay to detect serum antibody. E. coli cells that express foreign antigens on their surfaces can be used to develop antigen-specific immunoassays and to affinity purify monospecific antibodies.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6384191-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/6384191-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/6384191-6129241, http://linkedlifedata.com/resource/pubmed/commentcorrection/6384191-6132874, http://linkedlifedata.com/resource/pubmed/commentcorrection/6384191-6223920, http://linkedlifedata.com/resource/pubmed/commentcorrection/6384191-6305775, http://linkedlifedata.com/resource/pubmed/commentcorrection/6384191-6312563, http://linkedlifedata.com/resource/pubmed/commentcorrection/6384191-6325347, http://linkedlifedata.com/resource/pubmed/commentcorrection/6384191-6342093, http://linkedlifedata.com/resource/pubmed/commentcorrection/6384191-6368734, http://linkedlifedata.com/resource/pubmed/commentcorrection/6384191-6403984, http://linkedlifedata.com/resource/pubmed/commentcorrection/6384191-6818556, http://linkedlifedata.com/resource/pubmed/commentcorrection/6384191-7045877, http://linkedlifedata.com/resource/pubmed/commentcorrection/6384191-86314
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
160
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
199-203
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Legionella pneumophila surface antigens cloned and expressed in Escherichia coli are translocated to the host cell surface and interact with specific anti-Legionella antibodies.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.