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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1977-2-24
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pubmed:abstractText |
Mouse oocytes were found to synthesize proteins actively at the germinal vesicle, metaphase I, metaphase II, and pronuclear (6 hours post-fertilization) stages. The qualitative pattern components being synthesized in vitro, as demonstrated using polyacrylamide gel electrophoresis, changed throughout maturation and fertilization. Oocytes were arrested at metaphase I by greater than 0.1 mug/ml cycloheximide or actinomycin D. The protein pattern in oocytes cultured in the presence of actinomycin D progresses to a metaphase II pattern in spite of the nuclear maturation arrest, indicating a dissociation between meiotic maturation and the changes in the pattern of proteins synthesized at different stages of maturation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-104X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
198
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
337-42
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:63535-Animals,
pubmed-meshheading:63535-Cycloheximide,
pubmed-meshheading:63535-Dactinomycin,
pubmed-meshheading:63535-Female,
pubmed-meshheading:63535-Fertilization,
pubmed-meshheading:63535-Male,
pubmed-meshheading:63535-Meiosis,
pubmed-meshheading:63535-Mice,
pubmed-meshheading:63535-Oocytes,
pubmed-meshheading:63535-Ovum,
pubmed-meshheading:63535-Protein Biosynthesis
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pubmed:year |
1976
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pubmed:articleTitle |
Qualitative patterns of protein synthesis in the mouse oocyte.
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pubmed:publicationType |
Journal Article
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