Linked Life Data

6323434

Source:http://linkedlifedata.com/resource/pubmed/id/6323434

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1984-5-4
pubmed:abstractText
HeLa cells were made strictly dependent upon polyamine by growth in the presence of alpha-difluoromethylornithine, a specific inhibitor of ornithine decarboxylase. Under these conditions, the specific activity of the cellular polyamine pools eventually equilibrated to that of exogenously supplied [14C]putrescine; however, the process was very slow, requiring half-equilibration times of about 16 h for spermidine and 28 for spermine. Thus, the distribution of radioactivity in individual polyamines became a valid measure of polyamine content only after a continuous 4-day incorporation period. When propagated in polyamine-labeled cells, two picornaviruses were found to incorporate substantially different amounts of polyamine: about 0.6% of the cell pool for human rhinovirus 14 but only 0.04% for poliovirus. This content of polyamine was sufficient to neutralize nearly 27% of the negative charge of the RNA in human rhinovirus 14 but only 1.6% in poliovirus.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
259
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3639-43
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Radiochemical determination of polyamines in poliovirus and human rhinovirus 14.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't