Linked Life Data

6320168

Source:http://linkedlifedata.com/resource/pubmed/id/6320168

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-3-1
pubmed:abstractText
Muscarinic cholinergic receptors isolated from Drosophila heads, rat and human brain, dog heart, and monkey ciliary muscle were examined for structural similarities/differences by utilizing isoelectric focusing, sodium dodecyl sulfate/polyacrylamide gel electrophoresis, and monoclonal antibody crossreactivity. Muscarinic receptors were affinity labeled with [3H]propylbenzilylcholine mustard and subjected to isoelectric focusing. Muscarinic receptors from each species focused with an isoelectric point of 5.9. The same proteins all migrated with an apparent molecular mass of 80,000 daltons on sodium dodecyl sulfate gels. Six hybridomas secreting monoclonal antibodies specific for muscarinic receptors were developed by using purified rat brain muscarinic receptors as the antigen. The six different monoclonal antibodies immunoprecipitated muscarinic receptors from all tissues and species tested, including human and Drosophila brains, with equal efficacy. These data indicate that muscarinic receptors are highly conserved over a considerable evolutionary period. One of the six muscarinic receptor monoclonal antibodies also immunoprecipitated rat liver alpha 1-adrenergic receptors. Furthermore, two out of five monoclonal antibodies raised against alpha 1-receptors immunoprecipitated muscarinic receptors. These data suggest that some degree of structural homology exists between muscarinic cholinergic receptors and alpha 1-adrenergic receptors.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6320168-105086, http://linkedlifedata.com/resource/pubmed/commentcorrection/6320168-409619, http://linkedlifedata.com/resource/pubmed/commentcorrection/6320168-4153069, http://linkedlifedata.com/resource/pubmed/commentcorrection/6320168-4404457, http://linkedlifedata.com/resource/pubmed/commentcorrection/6320168-465889, http://linkedlifedata.com/resource/pubmed/commentcorrection/6320168-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/6320168-6261236, http://linkedlifedata.com/resource/pubmed/commentcorrection/6320168-6294103, http://linkedlifedata.com/resource/pubmed/commentcorrection/6320168-6308002, http://linkedlifedata.com/resource/pubmed/commentcorrection/6320168-6313709, http://linkedlifedata.com/resource/pubmed/commentcorrection/6320168-6766315, http://linkedlifedata.com/resource/pubmed/commentcorrection/6320168-6833279, http://linkedlifedata.com/resource/pubmed/commentcorrection/6320168-7055628, http://linkedlifedata.com/resource/pubmed/commentcorrection/6320168-7380005, http://linkedlifedata.com/resource/pubmed/commentcorrection/6320168-7463072
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
81
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
272-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Monoclonal antibodies detect the conservation of muscarinic cholinergic receptor structure from Drosophila to human brain and detect possible structural homology with alpha 1-adrenergic receptors.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't