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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1983-5-5
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pubmed:databankReference |
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pubmed:abstractText |
We have cloned a full-length 1.6-kilobase cDNA of a human mRNA coding for hypoxanthine phosphoribosyltransferase (HPRT; IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) into a simian virus 40-based expression vector and have determined its full nucleotide sequence. The inferred amino acid sequence agrees with a partial amino acid sequence determined for authentic human HPRT protein. Transfection of HPRT-deficient mouse LA9 cells with the purified plasmid leads to the expression of human HPRT enzyme activity in cells stably transfected and selected for enzyme activity in hypoxanthine/aminopterin/thymidine medium.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-1055375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-1103146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-118435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-173346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-210957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-232211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-291033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-357736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-364479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-369702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-4336693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-4611885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-4894690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-4952025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-5014925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-5231757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-569362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-5963888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-6020292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-6117931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-6173679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-6259625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-6269464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-6287227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-659426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-667015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-6791577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-6947270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-6952245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-6956912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-7074065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-7107641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6300847-861217
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
80
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
477-81
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6300847-Amino Acid Sequence,
pubmed-meshheading:6300847-Animals,
pubmed-meshheading:6300847-Base Sequence,
pubmed-meshheading:6300847-Cloning, Molecular,
pubmed-meshheading:6300847-DNA,
pubmed-meshheading:6300847-Humans,
pubmed-meshheading:6300847-Hypoxanthine Phosphoribosyltransferase,
pubmed-meshheading:6300847-L Cells (Cell Line),
pubmed-meshheading:6300847-Mice,
pubmed-meshheading:6300847-Plasmids,
pubmed-meshheading:6300847-RNA, Messenger,
pubmed-meshheading:6300847-Simian virus 40,
pubmed-meshheading:6300847-Transfection
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pubmed:year |
1983
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pubmed:articleTitle |
Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyl transferase.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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