Linked Life Data

6277978

Source:http://linkedlifedata.com/resource/pubmed/id/6277978

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1982-5-27
pubmed:abstractText
One hundred and fifteen faecal samples from children with gastroenteritis have been examined by solid-phase immune electron microscopy (SPIEM), standard direct electron microscopy and enzyme-linked immunosorbent assay (ELISA). Diluted high-titered anti-(human) rotavirus serum, produced in rabbit by immunization with purified pooled rotaviruses from gastroenteritis patients, was used as antiserum in the immunological tests. The presence of rotavirus was demonstrated in 41 (36%) of the 115 samples by both SPIEM and ELISA. No false positive reactions were seen. By direct electron microscopy rotavirus was detected in 35 (30%) of the specimens. In addition, other gastroenteritis viruses like adeno-, astro- and calicivirus were visualized by this non-specific test in eight samples. The applicability of these three methods in routine diagnostic work is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0166-0934
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
45-53
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Comparison of solid-phase immune electron microscopy, direct electron microscopy and enzyme-linked immunosorbent assay for detection of rotavirus in faecal samples.
pubmed:publicationType
Journal Article, Comparative Study